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Optical Review

, Volume 7, Issue 2, pp 119–122 | Cite as

Artifact Halo Reduction in Phase Contrast Microscopy Using Apodization

  • Tatsuro OtakiEmail author
OPTICAL SYSTEMS AND TECHNOLOGIES

Abstract

A new phase contrast microscopy technique for halo reduction is proposed. This technique is based on an apodization method combined with the Zernike phase contrast method. Although it has been a difficult theoretical problem, the proposed technique achieves halo reduction by considering angles of diffraction and phase differences. The technique utilizes an apodized phase plate consisting of a quarter wave phase shift ring with a 25% transmittance, and a pair of adjacent rings, which have 50% transmittance. This element is placed at the back focal plane of the objective. The result is startling, halo reduced images of phase objects providing enhanced inner details.

Key words

phase contrast microscopy apodization halo apodized phase plate angle of diffraction phase difference 

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References

  1. 1.
    A. Maréchal et M. Françon: Diffraction–Structure des Images (La Revue d’Optique Théorique et Instrumentale, Paris, 1960) Chap. 6, p. 71 (in French).Google Scholar
  2. 2.
    M. Born and E. Wolf: Principles of Optics (Pergamon Press, Oxford, 1980) 6th ed., p. 424.Google Scholar
  3. 3.
    F. A. Jenkins and H. E. White: Fundamentals of Optics (McGraw-Hill, New York, 1981) 4th ed., p. 602.Google Scholar
  4. 4.
    K. Yamamoto and A. Taira: J. Microscopy 129 (1983) 49.Google Scholar
  5. 5.
    Y. Hiramoto, S. Shimoda and Y. Shoji: Develop., Growth and Differ. 21 (1979) 141.Google Scholar
  6. 6.
    T. Asakura: OYO BUTURI 31 (1962) 730 (in Japanese).Google Scholar
  7. 7.
    T. Asakura: OYO BUTURI 32 (1963) 180 (in Japanese).Google Scholar
  8. 8.
    J. W. Goodman: Introduction to Fourier Optics (McGraw-Hill, New York, 1996) 2nd ed., p. 151.Google Scholar

Copyright information

© The Optical Society of Japan 2000

Authors and Affiliations

  1. 1.Optical Design Department, Instruments CompanyNikon CorporationYokohamaKanagawaJapan

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