Journal of Human Genetics

, Volume 53, Issue 2, pp 185–191 | Cite as

Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

  • Izuho HatadaEmail author
  • Sumiyo Morita
  • Mika Kimura
  • Takuro Horii
  • Riu Yamashita
  • Kenta Nakai
Short communication


Epigenetic regulation including DNA methylation plays an important role in several differentiation processes. We profiled global DNA methylation in the neural differentiation of P19 embryonic carcinoma cells using a microarray-based method called MIAMI. We found a genome-wide demethylation of genes. This suggests demethylation rather than methylation is important in neural differentiation.


Methylation Epigenetics Demethylation Genome-wide Profiling 



This study was supported in part by grants from the Japanese Science and Technology Agency, the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Ministry of Health, Labor, and Welfare of Japan.

Supplementary material

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Copyright information

© The Japan Society of Human Genetics and Springer 2008

Authors and Affiliations

  • Izuho Hatada
    • 1
    Email author
  • Sumiyo Morita
    • 1
  • Mika Kimura
    • 1
  • Takuro Horii
    • 1
  • Riu Yamashita
    • 2
  • Kenta Nakai
    • 2
  1. 1.Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular RegulationGunma UniversityMaebashiJapan
  2. 2.Laboratory of Functional Analysis in silico, Human Genome Center, Institute of Medical ScienceThe University of TokyoTokyoJapan

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