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Journal of Human Genetics

, Volume 53, Issue 1, pp 48–54 | Cite as

Glucose-6-phosphate dehydrogenase mutations in Mon and Burmese of southern Myanmar

  • Issarang Nuchprayoon
  • Chalisa Louicharoen
  • Warisa Charoenvej
Original Article

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is highly prevalent in Southeast Asians. G6PD mutations are associated with specific ethnic groups in Southeast Asia. Mon is a minority ethnic group in Myanmar, which speaks Monic, a distinct language of Mon-Khmer classification. We studied G6PD mutations in Mon and Burmese males of southern Myanmar who migrated to Thailand in Samutsakhon province. G6PD deficiency was identified in 19 (12%) of 162 Mon males and 17 (10%) of 178 Burmese males, and then assayed for G6PD mutations. Among 19 G6PD-deficient Mons, 12 were G6PD Mahidol; one case each was G6PD Jammu (871G > A; nt 1311C), G6PD Kaiping (1388G > A), G6PD Mediterranean (563C > T), a novel mutation 94(C > G); and three remain unidentified. Among 17 G6PD-deficient Burmese, 12 were G6PD Mahidol; one each was G6PD Coimbra (592C > T), G6PD Kerala-Kalyan (949G > A), and G6PD Valladolid (406C > T); and two remain unidentified. G6PD Mahidol (487G > A) is the most common mutation among Mons and Burmese. All G6PD deficient Mon and Burmese, except for a person with G6PD Valladolid, shared the same haplotype nt93T, nt1311C. Despite a similar language root with Cambodian’s Khmer language, our study suggests that Mon people share a common ancestry with Burmese rather than Cambodians.

Keywords

Glucose-6-phosphate dehydrogenase deficiency G6PD Mahidol G6PD Jammu Mon Burmese 

Notes

Acknowledgments

We thank Dr. Molee Wanichsuwan for his assistance in collecting blood samples at Samutsakhon Hospital. This research was supported by The Royal Golden Jubilee Ph.D. program of the Thailand Research Fund. We thank Allen Noble, PA, for editorial review of the manuscript.

References

  1. Ainoon O, Joyce J, Boo NY, Cheong SK, Zainal ZA, Hamidah NH (1999) Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Chinese. Hum Mutat 14:352–359PubMedCrossRefGoogle Scholar
  2. Ainoon O, Yu YH, Amir Muhriz AL, Boo NY, Cheong SK, Hamidah NH (2002) Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Malays. Hum Mutat 21:101–109CrossRefGoogle Scholar
  3. Betke K, Beutler E, Brewer GH, Kirkman HN, Luzzatto L, Motulsky AG, Ramot B, Siniscalco M (1967) Standardization of procedures for the study of glucose-6-phosphate dehydrogenase. Report of a WHO scientific group. WHO Tech Rep Ser No. 366Google Scholar
  4. Beutler E (1994) G6PD deficiency. Blood 84:3818–3838Google Scholar
  5. Beutler E, Kuhl W (1990) The NT 1311 polymorphism of G6PD: G6PD Mediterranean mutation may have originated independently in Europe and Asia. Am J Hum Genet 47:1008–1012PubMedGoogle Scholar
  6. Beutler E, Westwood Beryl, Kuhl W (1991) Definition of the mutations of G6PD Wayne, G6PD Viangchan, G6PD Jammu, and G6PD ‘LeJeune’. Acta Haematol 86:179–182PubMedCrossRefGoogle Scholar
  7. Church P (2003) A short history of Southeast Asia, 1st edn. Wiley, LondonGoogle Scholar
  8. Corcoran CM, Calabro V, Tamagnini G, Town M, Haider B, Vulliamy TJ, Mason PJ, Luzzatto L (1992) Molecular heterogeneity underlying the G6PD Mediterranean phenotype. Hum Genet 88:688–690PubMedCrossRefGoogle Scholar
  9. Flatz G, Sringram S (1963) Malaria and glucose-6-phosphate dehydrogenase deficiency in Thailand. Lancet 14:1248–1250CrossRefGoogle Scholar
  10. Gordon RG Jr (ed) (2005) Ethnologue: languages of the world, 15th edn. SIL International, Dallas. Online version: http://www.ethnologue.com/
  11. Hamel AR, Cabral IR, Sales TS, Costa FF, Olalla-Saad ST (2002) Molecular heterogeneity of G6PD deficiency in an Amazonian population and description of four new variants. Blood Cell Mol Dis 28:399–406CrossRefGoogle Scholar
  12. Huang CS, Hung KL, Huang MJ, Li YC, Liu TH, Tang TK (1996) Neonatal jaundice and molecular mutations in glucose-6-phosphate dehydrogenase deficient newborn infants. Am J Hematol 51:19–25PubMedCrossRefGoogle Scholar
  13. Iwai K, Hirono A, Matsuoka H, Kawamoto F, Horie T, Lin K, Tantular IS, Dachlan YP, Notopuro H, Hidayah NI, Salim AM, Fujii H, Miwa S, Ishii A (2001) Distribution of glucose-6-phosphate dehydrogenase mutations in Southeast Asia. Hum Genet 108: 445–449PubMedCrossRefGoogle Scholar
  14. Kawamoto F, Matsuoka H, Kanbe T,Tantular IS, Pusarawati S, Kerong HI, Damianus W, Mere D, Dachlan YP (2006) Further investigations of glucose-6-phosphate dehydrogenase variants in Flores Island, eastern Indonesia. J Hum Genet 51: 952–957PubMedCrossRefGoogle Scholar
  15. Laosombat V, Sattayasevana B, Janejindamai W, Viprakasit V, Shirakawa T, Nishiyama K, Matsuo M (2005) Molecular heterogeneity of glucose-6-phosphate dehydrogenase (G6PD) variants in the south of Thailand and identification of a novel variant (G6PD Songklanagarind). Blood Cell Mol Dis 34:191–196CrossRefGoogle Scholar
  16. Louicharoen C, Nuchprayoon I (2005) G6PD Viangchan (871G > A) is the most common G6PD-deficient variant in the Cambodian population. J Hum Genet 50:448–452PubMedCrossRefGoogle Scholar
  17. Martini G, Toniolo D, Vulliamy T, Luzzatto L, Dono R, Viglietto G, Paonessa G, D’Urso M, Persico MG (1986) Structure analysis of the X-linked gene encoding human glucose-6-phosphate dehydrogenase. EMBO J 5:1849–1855PubMedGoogle Scholar
  18. Matsuoka H, Nguon C, Kanbe T, Jalloh A, Sato H, Yoshida S, Hirai M, Arai M, Socheat D, Kawamoto F (2005) Glucose-6-phosphate dehydrogenase (G6PD) mutations in Cambodia: G6PD Viangchan (871G > A) is the most common variant in the Cambodian population. J Hum Genet 50:468–472PubMedCrossRefGoogle Scholar
  19. Matsuoka H, Wang J, Hirai M, Arai M, Yoshida S, Kobayashi T, Jalloh A, Lin K, Kawamoto F (2004) Glucose-6-phosphate dehydrogenase (G6PD) mutations in Myanmar: G6PD Mahidol (487G > A) is the most common variant in the Myanmar population. J Hum Genet 49:544–547PubMedCrossRefGoogle Scholar
  20. Ninokata A, Kimura R, Samakkarn U, Settheetham-Ishida W, Ishida T (2006) Coexistence of five G6PD variants indicates ethnic complexity of Phuket islanders, Southern Thailand. J Hum Genet 51:424–428PubMedCrossRefGoogle Scholar
  21. Nuchprayoon I, Sanpavat S, Nuchprayoon S (2002) Glucose-6-phosphate dehydrogenase (G6PD) mutations in Thailand: G6PD Viangchan (871G > A) is the most common deficiency variant in the Thai population. Hum Mutat 19:185PubMedCrossRefGoogle Scholar
  22. Poggi V, Town M, Foulkes NS, Luzzatto L (1990) Identification of a single base change in a new human mutant glucose-6-phosphate dehydrogenase gene by polymerase-chain-reaction amplification of the entire coding region from genomic DNA. Biochem J 271:157–160PubMedGoogle Scholar
  23. Saha S, Saha N, Tay JS, Jeyaseelan K, Basair JB, Chew SE (1994) Molecular characterization of red cell glucose-6-phosphate dehydrogenase deficiency in Singapore Chinese. Am J Hematol 47:273–277PubMedCrossRefGoogle Scholar
  24. Soemantri AG, Saha S, Saha N, Tay JSH (1995) Molecular variants of red cell glucose-6-phosphate dehydrogenase deficiency in Central Java, Indonesia. Hum Hered 45:346–350PubMedGoogle Scholar
  25. Sukumar S, Mukherjee MB, Colah RB, Mohanty D (2004) Molecular basis of G6PD deficiency in India. Blood Cells Mol Dis 33:141–145PubMedCrossRefGoogle Scholar
  26. Tang TK, Huang CS, Huang MJ, Tam KB, Yeh CH, Tang CJ (1992) Diverse point mutations result in glucose-6-phosphate dehydrogenase (G6PD) polymorphism in Taiwan. Blood 79:2135–2140PubMedGoogle Scholar
  27. Vaca G, Arambula E, Monsalvo A, Medina C, Nunez C, Sandoval L, Lopez-Guido B (2003) Glucose-6-phosphate dehydrogenase (G-6-PD) mutations in Mexico: four new G-6-PD variants. Blood Cells Mol Dis 31:112–120PubMedCrossRefGoogle Scholar
  28. Vives-Corrons JL, Zarza R, Aymerrich M, Bioxadera J, Carrera A, Colomer D (1997) Molecular analysis of glucose-6-phosphate dehydrogenase deficiency in Spain. Sangre (Barc) 42:391–398Google Scholar
  29. Vulliamy TJ, Othman A, Town M, Nathwani A, Falusi AG, Mason PJ, Luzzatto L (1991) Polymorphic sites in the African population detected by sequence analysis of the glucose-6-phosphate dehydrogenase gene outline the evolution of the variants A and A. Proc Natl Acad Sci USA 88:8568–8571PubMedCrossRefGoogle Scholar
  30. WHO Working Group (1989) Glucose-6-phosphate dehydrogenase deficiency. WHO Bull OMS 67:601–611Google Scholar
  31. Xu W, Westwood B, Bartsocas CS, Malcorra-Azpiazu JJ, Indrak K, Beutler E (1995) Glucose-6-phosphate dehydrogenase mutations and haplotypes in various ethnic groups. Blood 85:257–263PubMedGoogle Scholar
  32. Yan T, Cai R, Mo O, DongLin Z, Ouyan H, Huang L, Zhao M, Huang F, Li L, Liang X, Xu X (2006) Incidence and complete molecular characterization of glucose-6-phosphate dehydrogenase deficiency in the Guangxi Zhuang autonomous region of southern China: description of four novel mutations. Haematologica 91:1321–1328PubMedGoogle Scholar
  33. Zarza R, Pujades A, Rovira A, Saavedra R, Fernandez J, Aymerich M, Vives Corrons JL (1997) Two new mutations of the glucose-6-phosphate dehydrogenase (G6PD) gene associated with haemolytic anaemia: clinical, biochemical and molecular relationships. Br J Haematol 98:578–582PubMedCrossRefGoogle Scholar

Copyright information

© The Japan Society of Human Genetics and Springer 2007

Authors and Affiliations

  • Issarang Nuchprayoon
    • 1
    • 2
  • Chalisa Louicharoen
    • 2
  • Warisa Charoenvej
    • 3
  1. 1.Department of Pediatrics, Faculty of MedicineChulalongkorn UniversityBangkokThailand
  2. 2.Chula Medical Research Center, Faculty of MedicineChulalongkorn UniversityBangkokThailand
  3. 3.Department of Clinical PathologySamutsakhon HospitalSamutsakhonThailand

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