Skip to main content
SpringerLink
Log in

Cchobo, a hobo-related sequence in Ceratitis capitata

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

A hobo-related sequence, Cchobo, with high similarity to the Drosophila melanogaster HFL1 and hobo 108 elements was isolated from the medfly. Thirteen PCR-derived clones, which share 97.9–100% DNA identity, were sequenced, seven of which do not show frame-shift or stop codon mutations in their conceptual translations. The consensus sequence has 99.7% DNA identity with the D. melanogaster hobo element HFL1. In a phylogenetic analysis with other hobo-related elements, Cchobo clusters with the HFL1 and hobo 108 elements from D. melanogaster and hobo-related elements from D. simulans, D. mauritiana and Mamestra brassicae. These elements may have undergone horizontal transfer in the recent past. The genomic distribution of Cchobo was studied by FISH to mitotic and polytene chromosomes, which revealed that Cchobo is distributed within both the heterochromatin and euchromatin. Intra- and interstrain polymorphisms were detected both at euchromatic and heterochromatic sites. These findings suggest that active copies of the element may be present in the medfly genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • S.F. Altschul W. Gish W. Miller E.W. Myers D.J. Lipman (1990) ArticleTitleBasic local alignment search tool J Mol Biol 215 403–410 Occurrence Handle2231712 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D

    PubMed  CAS  Google Scholar 

  • P.W. Atkinson A.A. James (2002) ArticleTitleGermline transformants spreading out to many insect species Adv Gene 47 49–86 Occurrence Handle10.1016/S0065-2660(02)47002-2 Occurrence Handle1:CAS:528:DC%2BD38XjslGrsL8%3D

    Article  CAS  Google Scholar 

  • P.W. Atkinson W.D. Warren D.A. O’Brochta (1993) ArticleTitleThe hobo transposable element of Drosophila can be cross-mobilized in houseflies and excises like the Ac of maize Proc Natl Acad USA 90 9693–9697 Occurrence Handle1:CAS:528:DyaK2cXit1Wg

    CAS  Google Scholar 

  • B. Baker J. Schell H. Lorz N. Fedoroff (1986) ArticleTitleTransposition of maize controlling element ‘Activator’ in tobacco Proc Natl Acad Sci USA 83 4844–4848 Occurrence Handle16593722 Occurrence Handle1:CAS:528:DyaL28XksleltL0%3D

    PubMed  CAS  Google Scholar 

  • L. Baruffi G. Damiani C.R. Guglielmino C. Bandi A.R. Malacrida G. Gasperi (1995) ArticleTitlePolymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis data Heredity 74 425–437 Occurrence Handle7759289 Occurrence Handle1:CAS:528:DyaK2MXlvVehsro%3D

    PubMed  CAS  Google Scholar 

  • D.G. Bedo (1986) ArticleTitlePolytene and mitotic chromosome analysis in Ceratitis capitata (Diptera; Tephritidae) Can J Genet Cytol 28 180–188

    Google Scholar 

  • C Biémont G. Cizeron (1999) ArticleTitleDistribution of transposable elements in Drosophila species Genetica 105 43–62 Occurrence Handle10483093

    PubMed  Google Scholar 

  • F. Borsatti P. Azzoni M. Mandrioli (2003) ArticleTitleIdentification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera) Hereditas 139 151–155 Occurrence Handle15061816

    PubMed  Google Scholar 

  • I.A. Boussy G. Periquet (1993) The transposable element hobo in Drosophila melanogaster and related species JF McDonald (Eds) Transposable Elements and Evolution. Kluwer Academic Publisher The Netherlands 192–200

    Google Scholar 

  • I.A. Boussy M. Itoh (2004) ArticleTitleWanderings of hobo: a transposon in Drosophila melanogaster and its close relatives Genetica 120 125–136 Occurrence Handle15088653 Occurrence Handle1:CAS:528:DC%2BD2cXhsFylu78%3D

    PubMed  CAS  Google Scholar 

  • B.R. Calvi T.J. Hong S.D. Findley W.M. Gelbart (1991) ArticleTitleEvidence for a common evolutionary origin of inverted terminal repeat transposons in Drosophila and plants:hobo, Activator and Tam3 Cell 66 465–471 Occurrence Handle1651170 Occurrence Handle1:CAS:528:DyaK38Xkt1Whs70%3D

    PubMed  CAS  Google Scholar 

  • M. Carmena C. Gonzalez (1995) ArticleTitleTransposable elements map in a conserved pattern of distribution extending from beta-heterochromatin to centromeres in Drosophila melanogaster Chromosoma 103 676–684 Occurrence Handle7664614 Occurrence Handle1:STN:280:DyaK2Mzptlynsg%3D%3D

    PubMed  CAS  Google Scholar 

  • J.R. David P. Capy (1988) ArticleTitleGenetic variation of Drosophila melanogasternatural populations Trends Genet 4 106–111 Occurrence Handle3149056 Occurrence Handle1:STN:280:DyaL1M7osl2ltw%3D%3D

    PubMed  CAS  Google Scholar 

  • D.J. Finnegan (1989) ArticleTitleEukaryotic transposable elements and genome evolution Trends Genet 5 103–107 Occurrence Handle2543105 Occurrence Handle1:CAS:528:DyaK3cXhsVek

    PubMed  CAS  Google Scholar 

  • Fletcher, B.S., 1989. Life history strategies of tephritid fruit flies, pp. 195–208 in Fruit Flies: Their Biology, Natural Enemies and Control Vol 3B, edited by A.S. Robinson, G.H.S. Hooper. Elsevier, Amsterdam.

  • L.M. Gomulski C. Torti A.R. Malacrida G. Gasperi (1997) ArticleTitle Ccmar1, a full length mariner element from the Mediterranean fruit fly, Ceratitis capitata Insect Mol Biol 6 241–253 Occurrence Handle9272442 Occurrence Handle1:CAS:528:DyaK2sXlsVCrs7k%3D

    PubMed  CAS  Google Scholar 

  • L.M. Gomulski R.J. Pitts S. Costa G. Saccone C. Torti L.C. Polito G. Gasperi A.R. Malacrida F.C. Kafatos L.J. Zwiebel (2001) ArticleTitleGenomic organization and characterization of the white locus of the Mediterranean fruitfly Ceratitis capitata Genetics 157 1245–1255 Occurrence Handle11238408 Occurrence Handle1:CAS:528:DC%2BD3MXisFyktLg%3D

    PubMed  CAS  Google Scholar 

  • L.M. Gomulski C. Torti V. Murelli M. Bonizzoni G. Gasperi A.R. Malacrida (2004) ArticleTitleMedfly transposable elements: diversity, evolution, genomic impact and possible applications Insect Biochem Mol Biol 34 139–148 Occurrence Handle14871610 Occurrence Handle1:CAS:528:DC%2BD2cXhtFWisrk%3D

    PubMed  CAS  Google Scholar 

  • A.M. Handler S.P. Gomez (1995) ArticleTitleThe hobo transposable element has transposase dependent and independent excision activity in drosophilid species Mol Gen Genet 247 399–408 Occurrence Handle7770046 Occurrence Handle1:CAS:528:DyaK2MXmsVahsLY%3D

    PubMed  CAS  Google Scholar 

  • A.M. Handler S.P. Gomez (1996) ArticleTitleThe hobo transposable element excises and has related elements in Tephritid species Genetics 143 1339–1347 Occurrence Handle8807305 Occurrence Handle1:CAS:528:DyaK28XksFentL4%3D

    PubMed  CAS  Google Scholar 

  • J. Heringa (1999) ArticleTitleTwo strategies for sequence comparison: profile-preprocessed and secondary structure-induced multiple alignment Computers Chem 23 341–364 Occurrence Handle1:CAS:528:DyaK1MXktFKmt7k%3D

    CAS  Google Scholar 

  • M.G. Kidwell (1992) ArticleTitleHorizontal transfer of P elements and other short inverted repeat transposons Genetica 86 275–286 Occurrence Handle1334912 Occurrence Handle1:STN:280:DyaK3s7gs1Ciuw%3D%3D

    PubMed  CAS  Google Scholar 

  • M.G. Kidwell (1994) ArticleTitleThe evolutionary history of the P family of transposable elements J Hered 85 339–346 Occurrence Handle7963451 Occurrence Handle1:CAS:528:DyaK2MXhvFyit7o%3D

    PubMed  CAS  Google Scholar 

  • A. Koga A. Shimada A. Shima M. Sakaizumi H. Tachida H. Hori (2000) ArticleTitleEvidence for recent invasion of the medaka fish genome by the Tol2 transposable element Genetics 155 273–281 Occurrence Handle10790401 Occurrence Handle1:CAS:528:DC%2BD3cXjslKht7c%3D

    PubMed  CAS  Google Scholar 

  • J. Kwiatowski M. Krawczyk M. Jaworski D. Skarecky F.J. Ayala (1997) ArticleTitleErratic evolution of glycerol 3 phosphate dehydrogenase in Drosophila Chymomyza, and Ceratitis J Mol Evol 44 9–22 Occurrence Handle9010132 Occurrence Handle1:CAS:528:DyaK2sXlt1Wiug%3D%3D

    PubMed  CAS  Google Scholar 

  • A.R Lohe E.N. Moriyama E.N. Lidholm D.L. Hartl (1995) ArticleTitleHorizontal transmission, vertical inactivation and stochastic loss of mariner-like transposable elements Mol Biol Evol 12 62–72 Occurrence Handle7877497 Occurrence Handle1:CAS:528:DyaK2MXivVahtbg%3D

    PubMed  CAS  Google Scholar 

  • E.R. Lozovskaya D.I. Nurminsky D.L. Hartl T. Sullivan (1996) ArticleTitleGermline transformation of Drosophila virilis mediated by the transposable element hobo Genetics 142 173–177 Occurrence Handle8770594 Occurrence Handle1:CAS:528:DyaK28Xht1Cqtr0%3D

    PubMed  CAS  Google Scholar 

  • K. Maruyama D.L. Hartl (1991) ArticleTitleEvolution of the transposable element mariner in Drosophila species Genetics 128 319–329 Occurrence Handle1649069 Occurrence Handle1:CAS:528:DyaK3MXlt1ymu7o%3D

    PubMed  CAS  Google Scholar 

  • K. Michel A. Stamenova A.C. Pinkerton G. Franz A.S. Robinson A. Gariou-Papalexiou A. Zacharopoulou D.A. O’Brochta P.W. Atkinson (2001) ArticleTitle Hermes-mediated germ-line transformation of the Mediterranean fruit fly Ceratitis capitata Insect Mol Biol 10 155–162 Occurrence Handle11422511 Occurrence Handle1:CAS:528:DC%2BD3MXjtFKktLw%3D

    PubMed  CAS  Google Scholar 

  • D.A. O’Brochta W.D. Warren K.J. Saville P.W. Atkinson (1996) ArticleTitle Hermes, a functional non-Drosophilid insect gene vector from Musca domestica Genetics 142 907–914 Occurrence Handle8849896 Occurrence Handle1:CAS:528:DyaK28XitVGmtLo%3D

    PubMed  CAS  Google Scholar 

  • G. Periquet F. Lemeunier Y. Bigot M.H. Hamelin C. Bazin V. Ladeveze J. Eeken M.I. Galindo L. Pascual I. Boussy (1994) ArticleTitleThe evolutionary genetics of the hobo transposable element in the Drosophila melanogaster complex Genetica 93 79–90 Occurrence Handle7813919 Occurrence Handle1:CAS:528:DyaK2MXislOmsLc%3D

    PubMed  CAS  Google Scholar 

  • S. Pimpinelli M. Berloco L. Fanti P. Dimitri S. Bonaccorsi E. Marchetti R. Caiazzi C. Caggese M. Gatti (1995) ArticleTitleTransposable elements are stable components of Drosophila melanogaster heterochromatin Proc Natl Acad Sci USA 92 3804–3808 Occurrence Handle7731987 Occurrence Handle1:CAS:528:DyaK2MXlsVSlurY%3D

    PubMed  CAS  Google Scholar 

  • A.C. Pinkerton S. Whyard H.A. Mende C.J. Coates D.A. O’Brochta P.W. Atkinson (1999) ArticleTitleThe Queensland fruit fly, Bactrocera tryoni, contains multiple members of the hAT family of transposable elements Insect Mol Biol 8 423–434 Occurrence Handle10620037 Occurrence Handle1:CAS:528:DyaK1MXnvFOrs7c%3D

    PubMed  CAS  Google Scholar 

  • J.R. Powell A. Caccone J.M. Gleason L. Nigro (1993) ArticleTitleRates of DNA evolution in Drosophila depend on function and developmental stage of expression Genetics 133 291–298 Occurrence Handle8094697 Occurrence Handle1:CAS:528:DyaK3sXhvVyisr0%3D

    PubMed  CAS  Google Scholar 

  • G.M. Simmons (1992) ArticleTitleHorizontal transfer of hobo transposable elements within the Drosophila melanogaster species complex: evidence from DNA sequencing Mol Biol Evol 9 1050–1060 Occurrence Handle1331701 Occurrence Handle1:CAS:528:DyaK3sXktF2k

    PubMed  CAS  Google Scholar 

  • G.M. Simmons D. Plummer A. Simon I.A. Boussy J. Frantsve M. Itoh (1998) Horizontal and vertical transmission of hobo-related sequences between Drosophila melanogaster and Drosophila simulans M. Syvanen C.I. Kado (Eds) Horizontal Gene Transfer Chapman Hall New York 285–294

    Google Scholar 

  • S. Souames C. Bazin E. Bonnivard D. Higuet (2003) ArticleTitleBehaviour of the hobo transposable element with regard to TPE repeats in transgenic lines of Drosophila melanogaster Mol Biol Evol 20 2055–2066 Occurrence Handle12949153 Occurrence Handle1:CAS:528:DC%2BD2cXnt12gtw%3D%3D

    PubMed  CAS  Google Scholar 

  • E.M. Southern (1975) ArticleTitleDetection of specific sequences among DNA fragments separated by gel electrophoresis J Mol Biol 98 503–517 Occurrence Handle1195397 Occurrence Handle1:CAS:528:DyaE28XktVeltQ%3D%3D

    PubMed  CAS  Google Scholar 

  • R.D. Streck J.E. MacGaffey S.K. Beckendorf (1986) ArticleTitleThe structure of hobo elements and their site of insertion EMBO J 5 3615–3623 Occurrence Handle16453744 Occurrence Handle1:CAS:528:DyaL2sXhtFCgsbo%3D

    PubMed  CAS  Google Scholar 

  • P. Sundararajan P.W. Atkinson D.A. O’Brochta (1999) ArticleTitleTransposable element interactions in insects: cross mobilization of hobo and Hermes Insect Mol Biol 8 359–368 Occurrence Handle10469253 Occurrence Handle1:CAS:528:DyaK1MXmsVCmtro%3D

    PubMed  CAS  Google Scholar 

  • D.L. Swofford (1999) Phylogenetic Analysis Using Parsimony (*and other methods), Version 4 Sinauer Sunderland MA USA

    Google Scholar 

  • C. Torti A.R. Malacrida G. Yannopoulos C. Louis G. Gasperi (1994) ArticleTitleHybrid dysgenesis-like phenomena in the medfly, Ceratitis capitata (Diptera, Tephritidae) J Hered 85 92–99

    Google Scholar 

  • C. Torti L.M. Gomulski A.R. Malacrida P. Capy G. Gasperi (1997) ArticleTitleGenetic and molecular investigations on the endogenous mobile elements of nondrosophilid fruitflies Genetica 100 119–129 Occurrence Handle9440264 Occurrence Handle1:CAS:528:DyaK1cXkslWmtA%3D%3D

    PubMed  CAS  Google Scholar 

  • C. Torti L.M. Gomulski A.R. Malacrida P. Capy G. Gasperi (1998) ArticleTitleCharacterization and evolution of mariner elements from closely related species of fruit flies (Diptera: Tephritidae) J Mol Evol 46 288–298 Occurrence Handle9493354 Occurrence Handle1:CAS:528:DyaK1cXht1Slu7o%3D

    PubMed  CAS  Google Scholar 

  • C. Torti L.M. Gomulski D. Moralli E. Raimondi H.M. Robertson P. Capy G. Gasperi A.R. Malacrida (2000) ArticleTitleEvolution of different subfamilies of marinerelements within the medfly genome inferred from abundance and chromosomal distribution Chromosoma 108 523–532 Occurrence Handle10794574 Occurrence Handle1:CAS:528:DC%2BD3cXhslyrtro%3D

    PubMed  CAS  Google Scholar 

  • W.D. Warren P.W. Atkinson D.A. O’Brochta (1994) ArticleTitleThe Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-element of the hobo, Ac and Tam3 (hAT)element family Genet Res 64 87–97 Occurrence Handle7813905 Occurrence Handle1:CAS:528:DyaK2MXivFaitb8%3D Occurrence Handle10.1017/S0016672300032699

    Article  PubMed  CAS  Google Scholar 

  • W.D. Warren P.W. Atkinson D.A. O’Brochta (1995) ArticleTitleThe Australian bushfly, Musca vetustissima, contains a sequence related to the transposons of the hobo, Ac and Tam3 family Gene 154 133–134 Occurrence Handle7867942 Occurrence Handle1:CAS:528:DyaK2MXjvFyhs78%3D

    PubMed  CAS  Google Scholar 

  • S.D. Werman E.H. Davidson R.L. Britten (1990) ArticleTitleRapid evolution in a fraction of the Drosophilanuclear genome J Mol Evol 30 281–289 Occurrence Handle2109090 Occurrence Handle1:CAS:528:DyaK3cXhsFaqtLk%3D

    PubMed  CAS  Google Scholar 

  • U. Willhoeft G. Franz (1996) ArticleTitleIdentification of the sex-determining region of Ceratitis capitata Y chromosome by deletion mapping Genetics 144 737–745 Occurrence Handle8889534 Occurrence Handle1:CAS:528:DyaK28XmsVamsrw%3D

    PubMed  CAS  Google Scholar 

  • G. Yannopoulos N. Stamatis M. Monastrioti P. Hatzopoulos C. Louis (1987) ArticleTitle Hobo is responsible for the induction of hybrid dysgenesis by strains of Drosophila melanogaster bearing the male recombination factor 23.5MRF Cell 49 487–495 Occurrence Handle3032457 Occurrence Handle1:CAS:528:DyaL1cXltVCrtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • A. Zacharopoulou (1987) ArticleTitleCytogenetical analysis of mitotic and salivary gland chromosomes in the Medfly Ceratitis capitata Genome 29 67–71

    Google Scholar 

  • A. Zacharopoulou (1990) ArticleTitlePolytene chromosome map in the Medfly Ceratitis capitata Genome 33 184–197

    Google Scholar 

  • L.J. Zwiebel G. Saccone A. Zacharopoulou N.J. Besansky G. Favia F.H. Collins C. Louis F.C. Kafatos (1995) ArticleTitleThewhite gene of Ceratitis capitata: a phenotypic marker for germline transformation Science 270 2005–2008 Occurrence Handle8533095 Occurrence Handle1:CAS:528:DyaK28XltlSm

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal Biology, University of Pavia, Piazza Botta 9, I-27100, Pavia, Italy

    C. Torti, L. M. Gomulski, M. Bonizzoni, V. Murelli, D. Crisafulli, G. Gasperi & A. R. Malacrida

  2. Department of Genetics and Microbiology ‘A. Buzzati Traverso’, University of Pavia, Via Abbiategrasso 207, I-27100, Pavia, Italy

    D. Moralli & E. Raimondi

  3. Department of Genetics and Microbiology, University of Pavia, Pavia, Italy

    C. R. Guglielmino

  4. Laboratory of Populations, CNRS, Genetics and Evolution, 91198, Gif-sur-Yvette Cedex, France

    P. Capy

Authors
  1. C. Torti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. M. Gomulski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Bonizzoni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. Murelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Moralli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. R. Guglielmino
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. Raimondi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. D. Crisafulli
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. P. Capy
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. G. Gasperi
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. A. R. Malacrida
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. R. Malacrida.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Torti, C., Gomulski, L.M., Bonizzoni, M. et al. Cchobo, a hobo-related sequence in Ceratitis capitata . Genetica 123, 313–325 (2005). https://doi.org/10.1007/s10038-004-7126-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10038-004-7126-5

Keywords

Search

Navigation