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Hernia

, Volume 21, Issue 4, pp 643–648 | Cite as

Burst inflation test for measuring biomechanical properties of rat abdominal walls

  • V. D. Mahalingam
  • B. C. Syverud
  • A. M. Myers
  • K. W. VanDusen
  • L. M. Larkin
  • W. M. Kuzon
  • E. M. Arruda
Original Article

Abstract

Purpose

Evaluation of potential grafts to improve upon current strategies for abdominal wall (AW) repair in small animal models typically involves mechanical testing using methods that currently are inadequate to assess physiologically relevant parameters. This study introduces burst inflation testing as a more relevant assessment of the mechanical integrity of the AW compared to traditional tensile testing.

Methods

AWs were excised from 14 healthy adult Fischer 344 rats and tested using either a custom burst inflation device or an Instron tensile testing system. Modulus outcomes from both testing methods were compared.

Results

Mechanical analyses of native AW using burst and tensile testing methods resulted in similar average tissue moduli, but with the burst test, there was significantly less variability among specimens.

Conclusions

The burst test had greater repeatability compared to tensile testing and has the ability to test repaired AWs without compromising the integrity of the repair site, making it a useful tool for assessing graft repairs.

Keywords

Incisional hernia Burst inflation Tensile strength Mechanical test Modulus 

Notes

Acknowledgements

Funding provided by the Department of Surgery, Section of Plastic Surgery at University of Michigan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Human and animal rights

All procedures performed involving animals were in accordance with the ethical standards of the University Committee for the Use and Care of Animals at the University of Michigan (PRO00005146). This article does not contain any studies with human participants performed by any of the authors.

Informed consent

None.

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Copyright information

© Springer-Verlag France 2016

Authors and Affiliations

  • V. D. Mahalingam
    • 1
    • 2
  • B. C. Syverud
    • 3
  • A. M. Myers
    • 1
  • K. W. VanDusen
    • 2
  • L. M. Larkin
    • 2
    • 3
  • W. M. Kuzon
    • 1
    • 4
  • E. M. Arruda
    • 3
    • 5
  1. 1.Section of Plastic Surgery, Department of SurgeryUniversity of MichiganAnn ArborUSA
  2. 2.Department of Molecular and Integrative PhysiologyUniversity of MichiganAnn ArborUSA
  3. 3.Department of Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  4. 4.Ann Arbor Veterans Administration Medical CenterAnn ArborUSA
  5. 5.Department of Mechanical Engineering, Program in Macromolecular Science and EngineeringUniversity of MichiganAnn ArborUSA

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