Burst inflation test for measuring biomechanical properties of rat abdominal walls
Evaluation of potential grafts to improve upon current strategies for abdominal wall (AW) repair in small animal models typically involves mechanical testing using methods that currently are inadequate to assess physiologically relevant parameters. This study introduces burst inflation testing as a more relevant assessment of the mechanical integrity of the AW compared to traditional tensile testing.
AWs were excised from 14 healthy adult Fischer 344 rats and tested using either a custom burst inflation device or an Instron tensile testing system. Modulus outcomes from both testing methods were compared.
Mechanical analyses of native AW using burst and tensile testing methods resulted in similar average tissue moduli, but with the burst test, there was significantly less variability among specimens.
The burst test had greater repeatability compared to tensile testing and has the ability to test repaired AWs without compromising the integrity of the repair site, making it a useful tool for assessing graft repairs.
KeywordsIncisional hernia Burst inflation Tensile strength Mechanical test Modulus
Funding provided by the Department of Surgery, Section of Plastic Surgery at University of Michigan.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Human and animal rights
All procedures performed involving animals were in accordance with the ethical standards of the University Committee for the Use and Care of Animals at the University of Michigan (PRO00005146). This article does not contain any studies with human participants performed by any of the authors.
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