Pediatric and Developmental Pathology

, Volume 7, Issue 5, pp 453–458

Characterization of Inflammation in Syphilitic Villitis and in Villitis of Unknown Etiology

  • Payal Kapur
  • Dinesh Rakheja
  • Ana M. Gomez
  • Jeanne Sheffield
  • Pablo Sanchez
  • Beverly Barton Rogers
Original article

Abstract

Chronic villitis is a histologic diagnosis that may be either associated with infection, or termed villitis of undetermined etiology (VUE). The lymphocytic infiltrate in VUE has been reported to consist of maternal lymphocytes, but the origin of the lymphocytic infiltrate in infectious villitis has not been identified. The purpose of our study was to compare the maternal vs. fetal origin of the infiltrating lymphocytes in VUE and syphilitic villitis, and to expand the immunophenotypic data provided by previous studies. Paraffin-embedded placentas from four males with VUE and two males with syphilitic villitis were subjected to fluorescence in situ hybridization (FISH) for the X and Y chromosomes. Serial sections were stained with antibodies to CD3, CD4, CD8, CD68, HLA-DR, and CD20. Quantitation of the relative number of cells marking with each antibody was done for four villi in each slide. CD3 lymphocytes predominated in both VUE and syphilitic villitis, with slightly more CD8 cells compared to CD4 cells. CD68 and HLA-DR positive cells were as frequent as CD3 cells, and B-lymphocytes were rare. Maternal cells were the predominant intravillous population in both VUE and syphilitic villitis, and neutrophils in syphilitic villitis were also maternal. These data indicate that the immune response in both syphilitic villitis and VUE is similar, raising the possibility of a similar immunopathogenetic pathway.

chronic villitis syphilis villitis of undetermined etiology 

References

  1. 1.
    Altshuler, G, Russell, P 1975The human placental villitides: a review of chronic intrauterine infectionCurr Top Pathol6064112PubMedGoogle Scholar
  2. 2.
    Ruschoff, J, Boger, A, Zwiens, G 1985Chronic placentitis—a clinicopathological studyArch Gynecol2371925CrossRefPubMedGoogle Scholar
  3. 3.
    Benirschke, K, Kaufmann, P 2000Infectious Diseases. Pathology of the Human PlacentaSpringer USNew York653659CrossRefGoogle Scholar
  4. 4.
    Hyde, SR, Altshuler, G 1999

    Infectious disorders of the placenta

    Lewis, STPerrin, E eds. Pathology of the PlacentaChurchill LivingstonePhiladelphia329332
    Google Scholar
  5. 5.
    Redline, RW, Patterson, P 1993Villitis of unknown etiology is associated with major infiltration of fetal tissue by maternal inflammatory cellsAm J Pathol143473479PubMedPubMedCentralGoogle Scholar
  6. 6.
    Labarrere, CA, Faulk, WP 1995Maternal cells in chorionic villi from placentae of normal and abnormal human pregnanciesAm J Reprod Immunol335459CrossRefPubMedGoogle Scholar
  7. 7.
    Labarrere, C, Althabe, O 1985Chronic villitis of unknown etiology and maternal arterial lesions in preeclamptic pregnanciesEur J Obstet Gynecol Reprod Biol20111CrossRefPubMedGoogle Scholar
  8. 8.
    Labarrere, C, Althabe, O, Caletti, E, Muscolo, D 1986Deficiency of blocking factors in intrauterine growth retardation and its relationship with chronic villitisAm J Reprod Immunol Microbiol101419CrossRefPubMedGoogle Scholar
  9. 9.
    Russell, P 1980Inflammatory lesions of the human placenta. III. The histopathology of villitis of unknown aetiologyPlacenta1227244CrossRefPubMedGoogle Scholar
  10. 10.
    Altshuler, G, Russell, P, Ermocilla, R 1975The placental pathology of small-for-gestational age infantsAm J Obstet Gynecol121351359CrossRefPubMedGoogle Scholar
  11. 11.
    Labarrere, C, Althabe, O, Telenta, M 1982Chronic villitis of unknown aetiology in placentae of idiopathic small for gestational age infantsPlacenta3309317CrossRefPubMedGoogle Scholar
  12. 12.
    Althabe, O, Labarrere, C 1985Chronic villitis of unknown aetiology and intrauterine growth-retarded infants of normal and low ponderal indexPlacenta6369373CrossRefPubMedGoogle Scholar
  13. 13.
    Labarrere, C, Althabe, O 1987Chronic villitis of unknown aetiology in recurrent intrauterine fetal growth retardationPlacenta8167173CrossRefPubMedGoogle Scholar
  14. 14.
    Labarrere, CA, Catoggio, LJ, Mullen, EG, Althabe, OH 1986Placental lesions in maternal autoimmune diseasesAm J Reprod Immunol Microbiol127886CrossRefPubMedGoogle Scholar
  15. 15.
    Altemani, AM 1992Immunohistochemical study of the inflammatory infiltrate in villitis of unknown etiology. A qualitative and quantitative analysisPathol Res Pract188303309CrossRefPubMedGoogle Scholar
  16. 16.
    Labarrere, CA, McIntyre, JA, Faulk, WP 1990Immunohistologic evidence that villitis in human normal term placentas is an immunologic lesionAm J Obstet Gynecol162515522CrossRefPubMedGoogle Scholar
  17. 17.
    Carlson D, Soslow R, Baergen RN. Immunophenotyping of villitis of unknown etiology (Abstract). Mod Pathol 2000; 31:203A.Google Scholar
  18. 18.
    Altemani, AM, Bittencourt, AL, Lana, AM 2000Immunohistochemical characterization of the inflammatory infiltrate in placental Chagas’ disease: a qualitative and quantitative analysisAm J Trop Med Hyg62319324PubMedGoogle Scholar
  19. 19.
    Greco, MA, Wieczorek, R, Sachdev, R, Kaplan, C, Nuovo, GJ, Demopoulos, RI 1992Phenotype of villous stromal cells in placentas with cytomegalovirus, syphilis, and nonspecific villitisAm J Pathol141835842PubMedPubMedCentralGoogle Scholar

Copyright information

© Society for Pediatric Pathology 2004

Authors and Affiliations

  • Payal Kapur
    • 1
  • Dinesh Rakheja
    • 1
  • Ana M. Gomez
    • 1
  • Jeanne Sheffield
    • 2
  • Pablo Sanchez
    • 3
  • Beverly Barton Rogers
    • 1
  1. 1.Department of PathologyThe University of Texas Southwestern Medical SchoolDallasUSA
  2. 2.Department of Obstetrics and GynecologyThe University of Texas Southwestern Medical SchoolDallasUSA
  3. 3.Department of PediatricsThe University of Texas Southwestern Medical SchoolDallasUSA

Personalised recommendations