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Ecosystems

, Volume 21, Issue 5, pp 901–912 | Cite as

Modest Gaseous Nitrogen Losses Point to Conservative Nitrogen Cycling in a Lowland Tropical Forest Watershed

  • Fiona M. Soper
  • Phillip G. Taylor
  • William R. Wieder
  • Samantha R. Weintraub
  • Cory C. Cleveland
  • Stephen Porder
  • Alan R. Townsend
Article

Abstract

Primary tropical rainforests are generally considered to be relatively nitrogen (N) rich, with characteristically large hydrologic and gaseous losses of inorganic N. However, emerging evidence suggests that some tropical ecosystems can exhibit tight N cycling, with low biologically available losses. In this study, we combined isotopic data with a well-characterized watershed N mass balance to close the N budget and characterize gaseous N losses at the ecosystem scale in a lowland tropical rainforest on the Osa Peninsula in southwestern Costa Rica. We measured δ15N and δ18O of nitrate (NO3 ) in precipitation, surface, shallow and deep soil lysimeters and stream water biweekly for 1 year. Enrichment of both isotopes indicates that denitrification occurs predominantly as NO3 moves from surface soil down to 15 cm depth or laterally to stream water, with little further processing in deeper soil. Two different isotopic modeling approaches suggested that the gaseous fraction comprises 14 or 32% of total N loss (2.7 or 7.5 kg N ha−1 y−1), though estimates are sensitive to selection of isotopic fractionation values. Gas loss estimates using the mass balance approach (3.2 kg N ha−1 y−1) fall within this range and include N2O losses of 0.9 kg N ha−1 y−1. Overall, gaseous and soluble hydrologic N losses comprise a modest proportion (~ 25%) of the total N inputs to this ecosystem. By contrast, relatively large, episodic hydrologic losses of non-biologically available particulate N balance the majority of N inputs and may contribute to maintaining conservative N cycling in this lowland tropical forest. Similar patterns of N cycling may occur in other tropical forests with similar state factor combinations—high rainfall, steep topography, relatively fertile soils—such as the western arc of the Amazon Basin and much of IndoMalaysia, but this hypothesis remains untested.

Keywords

denitrification nitrogen cycle nitrous oxide δ15δ18NO3 tropical forest 

Notes

Acknowledgements

We thank W. Combronero-Castro for his invaluable assistance with fieldwork in Costa Rica. We thank M. Jimenez and the late H. Michaud of the Drake Bay Wilderness Camp for providing field access and logistical support and we also thank F. Campos Rivera, the Organizaciόn para Estudios Tropicales (OET) and the Ministerio de Ambiente y Energia (MINAE) for assisting with research permits and logistics in Costa Rica. This study was supported by a National Science Foundation GK-12 fellowship, the Andrew Mellon Foundation and an NSF DEB Award (# 0919080) to CC and NSF DEB Award (# 0918387) to SP.

Supplementary material

10021_2017_193_MOESM1_ESM.docx (109 kb)
Supplementary material 1 (DOCX 109 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Fiona M. Soper
    • 1
  • Phillip G. Taylor
    • 2
  • William R. Wieder
    • 3
  • Samantha R. Weintraub
    • 4
  • Cory C. Cleveland
    • 1
  • Stephen Porder
    • 5
  • Alan R. Townsend
    • 2
  1. 1.Department of Ecosystem and Conservation SciencesUniversity of MontanaMissoulaUSA
  2. 2.The Institute of Artic and Alpine ResearchUniversity of ColoradoBoulderUSA
  3. 3.Climate and Global Dynamics LaboratoryNational Center for Atmospheric ResearchBoulderUSA
  4. 4.National Ecological Observatory NetworkBoulderUSA
  5. 5.Department of Ecology and Evolutionary BiologyBrown UniversityProvidenceUSA

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