, Volume 20, Issue 6, pp 1073–1088 | Cite as

Interactive Effects of Vegetation Type and Topographic Position on Nitrogen Availability and Loss in a Temperate Montane Ecosystem

  • Samantha R. WeintraubEmail author
  • Paul D. Brooks
  • Gabriel J. Bowen


Determining the fate of deposited nitrogen (N) in natural ecosystems remains a challenge. Heterogeneity of vegetation types and resulting plant–soil feedbacks interact with topo-hydrologic gradients to mediate spatial patterns of N availability and loss, yet net effects of variation in these two factors together across complex terrain remain unclear. Here we measured a suite of N-cycle pools and fluxes in sites that differed factorially in vegetation type (mixed forest vs. herbaceous) and topographic position (upslope vs. downslope) in a protected montane watershed near Salt Lake City, UT. Vegetation type was associated with large variation in N availability—herbaceous sites had larger NO3 pools, higher NO3 :NH4 + ratios, higher nitrification potentials, lower soil C:N values, enriched δ15N values, and lower microbial biomass compared to forests, especially those upslope. Downslope sites tended to exhibit higher N availability and indicators of N-cycle openness, but patterns were moderated by vegetation type. In downslope forest, soil NO3 depth profiles and higher foliar N content suggested trees were accessing deep soil N and transferring it to the surface via litterfall, while more deep soil NO3 but no change in surface or foliar N suggested herbaceous cover was not N limited or deeper N pools were not accessible. Soil NO3 leaching from below the rooting zone closely tracked N availability, revealing a link between N status and hydrologic loss as well as an important role for roots in N retention. NO3 isotopes did not reveal a similar link for gaseous losses (that is, denitrification), instead reflecting nitrification and/or transport dynamics. Together, these results suggest a coupled ecological, topo-hydrologic perspective can help assess the fate of N in complex landscapes.


soil nitrogen topographic wetness nitrate leaching plant–soil interactions 



We are grateful to Suvankar Chakraborty for assistance with stable isotope analyses, and to Christina Woltz, Dave Eiriksson, Steven Hall, Brett Boyer, Simone Jackson, Rachel Gabor, and Mallory Millington for their help with field and laboratory work. Funding provided by US National Science Foundation Grant DBI-1337947 was awarded to G. Bowen, and EPSCoR Grant IIA 1208732 was awarded to Utah State University. Friends of Red Butte Creek at the University of Utah also provided funds that supported this work.


  1. Adams HR, Barnard HR, Loomis AK. 2014. Topography alters tree growth–climate relationships in a semi-arid forested catchment. Ecosphere 5:148.CrossRefGoogle Scholar
  2. Asano Y, Compton JE, Church MR. 2006. Hydrologic flowpaths influence inorganic and organic nutrient leaching in a forest soil. Biogeochemistry 81:191–204.CrossRefGoogle Scholar
  3. Bell MD, Sickman JO. 2014. Correcting for background nitrate contamination in KCl-extracted samples during isotopic analysis of oxygen and nitrogen by the denitrifier method. Rapid Commun Mass Spectrom 28:520–6.CrossRefPubMedGoogle Scholar
  4. Belnap J, Welter JR, Grimm NB, Barger N, Ludwig JA. 2005. Linkages between microbial and hydrologic processes in arid and semiarid watersheds. Ecology 86:298–307.CrossRefGoogle Scholar
  5. Biederman JA, Meixner T, Harpold AA, Reed DE, Gutmann ED, Gaun JA, Brooks PD. 2016. Riparian zones attenuate nitrogen loss following bark beetle-induced lodgepole pine mortality. J Geophys Res. doi: 10.1002/2015JG003284.Google Scholar
  6. Bingham AH, Cotrufo MF. 2016. Organic nitrogen storage in mineral soil: implications for policy and management. Sci Total Environ 551–552:116–26.CrossRefPubMedGoogle Scholar
  7. Binkley D. 1984. Ion exchange resin bags: factors affecting estimates of nitrogen availability. Soil Sci Soc Am J 48:1181–4.CrossRefGoogle Scholar
  8. Binkley D, Sollins P, Bell R, Sachs D, Myrold D. 1992. Biogeochemistry of adjacent conifer and alder-conifer stands. Ecology 73:2022–33.CrossRefGoogle Scholar
  9. Bohlen P, Groffman P, Driscoll C, Fahey T. 2001. Plant-soil-microbial interactions in a northern hardwood forest. Ecology 82:965–78.Google Scholar
  10. Brooks PD, Williams MW, Schmidt SK. 1998. Inorganic nitrogen and microbial biomass dynamics before and during spring snowmelt. Biogeochemistry 43:1–15.CrossRefGoogle Scholar
  11. Brooks PD, Campbell DH, Tonnessen KA, Heuer K. 1999. Natural variability in N export from headwater catchments: snow cover controls on ecosystem N retention. Hydrol Process 13:2191–201.CrossRefGoogle Scholar
  12. Brooks JR, Barnard HR, Coulombe R, McDonnell JJ. 2010. Ecohydrologic separation of water between trees and streams in a Mediterranean climate. Nat Geosci 3:100–4.CrossRefGoogle Scholar
  13. Casciotti KL, Sigman DM, Hastings MG, Böhlke JK, Hilkert A. 2002. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method. Anal Chem 74:4905–12.CrossRefPubMedGoogle Scholar
  14. Chapman SK, Langley JA, Hart SC, Koch GW. 2006. Plants actively control nitrogen cycling: uncorking the microbial bottleneck. New Phytol 169:27–34.CrossRefPubMedGoogle Scholar
  15. Clark CM, Tilman D. 2008. Loss of plant species after chronic low-level nitrogen deposition to prairie grasslands. Nature 451:712–15.CrossRefPubMedGoogle Scholar
  16. Cohen WB, Yang Z, Stehman SV, Schroeder TA, Bell DM, Masek JG, Huang C, Meigs GW. 2016. Forest disturbance across the conterminous United States from 1985-2012: the emerging dominance of forest decline. For Ecol Manag 360:242–52.CrossRefGoogle Scholar
  17. Corbin JD, D’Antonio CM. 2004. Effects of exotic species on soil nitrogen cycling: Implications for restoration. Weed Technol 18:1464–7.CrossRefGoogle Scholar
  18. Creed IF, Band LE, Foster NW, Morrison IK, Nicolson JA, Semkin RS, Jeffries DS. 1996. Regulation of nitrate-N release from temperate forests: a test of the N flushing hypothesis. Water Resour Res 32:3337.CrossRefGoogle Scholar
  19. Davidson EA, de Carvalho CJR, Figueira AM, Ishida FY, Ometto JPHB, Nardoto GB, Sabá RT, Hayashi SN, Leal EC, Vieira ICG, Martinelli LA. 2007. Recuperation of nitrogen cycling in Amazonian forests following agricultural abandonment. Nature 447:995–8.CrossRefPubMedGoogle Scholar
  20. Ehleringer JR, Arnow LA, Arnow T, McNulty IB, Negus NC. 1992. Red Butte Canyon Research Natural Area: history, flora, geology, climate and ecology. Great Basin Nat 52:95–121.Google Scholar
  21. Fenn ME, Haeuber R, Tonnesen GS, Baron JS, Grossman-Clarke S, Hope D, Jaffe DA, Copeland S, Geiser L, Rueth HM, Sickman JO. 2003. Nitrogen emissions, deposition, and monitoring in the western United States. BioScience 53:391–403.CrossRefGoogle Scholar
  22. Fierer N, Strickland M, Liptzin D, Bradford M, Cleveland C. 2009. Global patterns in belowground communities. Ecol Lett 12:1238–49.CrossRefPubMedGoogle Scholar
  23. Galloway JN, Aber JD, Erisman JW, Seitzinger SP, Howarth RW, Cowling EB, Cosby BJ. 2003. The nitrogen cascade. BioScience 53:341–56.CrossRefGoogle Scholar
  24. Gaskin JW, Dowd JF, Nutter WL, Swank WT. 1989. Vertical and lateral components of soil nutrient flux in a hillslope. J Environ Qual 18:403–10.CrossRefGoogle Scholar
  25. Good SP, Noone D, Bowen G. 2015. Hydrologic connectivity constrains partitioning of global terrestrial water fluxes. Science 349:175–7. doi: 10.1126/science.aaa5931.CrossRefPubMedGoogle Scholar
  26. Gregorich E, Wen G, Voroney R, Kachanoski R. 1990. Calibration of a rapid direct chloroform extraction method for measuring soil microbial biomass C. Soil Biol Biochem 22:1009–11.CrossRefGoogle Scholar
  27. Gruber N, Galloway JN. 2008. An Earth-system perspective of the global nitrogen cycle. Nature 451:293–6.CrossRefPubMedGoogle Scholar
  28. Hall SJ, Ogata EM, Weintraub SR, Baker MA, Ehleringer JR, Czimczik CI, Bowling DR. 2016a. Convergence in nitrogen deposition and cryptic isotopic variation across urban and agricultural valleys in northern Utah. JGR Biogeosci. doi: 10.1002/2016JG003354.Google Scholar
  29. Hall SJ, Weintraub SR, Bowling DR. 2016b. Scale-dependent linkages between nitrate isotopes and denitrification in surface soils: implications for isotope measurements and models. Oecologia 181:1–11.CrossRefGoogle Scholar
  30. Hall SJ, Weintraub SR, Eiriksson D, Brooks PD, Baker MA, Bowen GJ, Bowling DR. 2016c. Stream nitrogen inputs reflect groundwater across a snowmelt-dominated montane to urban watershed. Environ Sci Technol 50:1137–46.CrossRefPubMedGoogle Scholar
  31. Hart S, Gunther A. 1989. In situ estimates of annual net nitrogen mineralization and nitrification in a subarctic watershed. Oecologia 80:284–8.CrossRefPubMedGoogle Scholar
  32. Hinckley E, Barnes R, Anderson S, Williams M, Bernasconi S. 2014. Nitrogen retention and transport differ by hillslope aspect at the rain-snow transition of the Colorado Front Range. J Geophys Res 119:1281–96.CrossRefGoogle Scholar
  33. Huang S, Arain MA, Arora VK, Yuan F, Brodeur J, Peichl M. 2011. Analysis of nitrogen controls on carbon and water exchanges in a conifer forest using the CLASS-CTEM N+ model. Ecol Model 222:3743–60.CrossRefGoogle Scholar
  34. Huber C. 2005. Long lasting nitrate leaching after bark beetle attack in the highlands of the Bavarian Forest National Park. J Environ Qual 34:1772–9.CrossRefPubMedGoogle Scholar
  35. Lee MR, Flory SL, Phillips RP. 2012. Positive feedbacks to growth of an invasive grass through alteration of nitrogen cycling. Oecologia 170:457–65.CrossRefPubMedGoogle Scholar
  36. Lehto T, Zwiazek JJ. 2011. Ectomycorrhizas and water relations of trees: a review. Mycorrhiza 21:71–90.CrossRefPubMedGoogle Scholar
  37. Lewis DB, Catellano MJ, Kaye JP. 2014. Forest succession, soil carbon accumulation, and rapid nitrogen storage in poorly remineralized soil organic matter. Ecology 95:2687–93.CrossRefGoogle Scholar
  38. Liu X, Duan L, Mo J, Du E, Shen J, Lu X, Zhang Y, Zhou X, He C, Zhang F. 2011. Nitrogen deposition and its ecological impact in China: an overview. Environ Pollut 159:2251–64.CrossRefPubMedGoogle Scholar
  39. Lohse KA, Dietrich WE. 2005. Contrasting effects of soil development on hydrological properties and flow paths. Water Resour Res 41:W12419. doi: 10.1029/2004WR003403.CrossRefGoogle Scholar
  40. Lohse KA, Brooks PD, McIntosh JC, Meixner T, Huxman TE. 2009. Interactions between biogeochemistry and hydrologic systems. Annu Rev Environ Resour 34:65–96.CrossRefGoogle Scholar
  41. Lovett GM, Goodale CL. 2011. A new conceptual model of nitrogen saturation based on experimental nitrogen addition to an oak forest. Ecosystems 14:615–31.CrossRefGoogle Scholar
  42. Lovett GM, Weathers KC, Arthur MA. 2002. Control of nitrogen loss from forested watersheds by soil carbon: nitrogen ratio and tree species composition. Ecosystems 5:712–18.CrossRefGoogle Scholar
  43. Lü XT, Freschet GT, Flynn DFB, Han XG. 2012. Plasticity in leaf and stem nutrient resorption proficiency potentially reinforces plant-soil feedbacks and microscale heterogeneity in a semi-arid grassland. J Ecol 100:144–50.CrossRefGoogle Scholar
  44. Mariotti A, Germon JC, Hubert P, Kaiser P, Letolle R, Tardieux A, Tardieux P. 1981. Experimental determination of nitrogen kinetic isotope fractionation: some principles; illustration for the denitrification and nitrification processes. Plant Soil 62:413–30.CrossRefGoogle Scholar
  45. McClain ME, Boyer EW, Dent CL, Gergel SE, Grimm NB, Groffman PM, Hart SC, Harvey JW, Johnston CA, Mayorga E, McDowell WH, Pinay G. 2003. Biogeochemical hot spots and hot moments at the interface of terrestrial and aquatic ecosystems. Ecosystems 6:301–12.CrossRefGoogle Scholar
  46. Nadelhoffer KJ, Emmett BA, Gundersen P, Kjønaas OJ, Koopmans CJ, Schleppi P, Tietema A, Wright RF. 1999. Nitrogen deposition makes a minor contribution to carbon sequestration in temperate forests. Nature 398:145–8.CrossRefGoogle Scholar
  47. Nanus L, Clow DW, Saros JE, Stephens VC, Campbell DH. 2012. Mapping critical loads of nitrogen deposition for aquatic ecosystems in the Rocky Mountains, USA. Environ Pollut 166:125–35.CrossRefPubMedGoogle Scholar
  48. Ollinger SV, Smith ML. 2005. Net primary production and canopy nitrogen in a temperate forest landscape: an analysis using imaging spectroscopy, modeling and field data. Ecosystems 8:760–78.CrossRefGoogle Scholar
  49. Perakis SS, Sinkhorn ER. 2011. Biogeochemistry of a temperate forest nitrogen gradient. Ecology 92:1481–91.CrossRefPubMedGoogle Scholar
  50. Perakis SS, Matkins JJ, Hibbs DE. 2012. N2-fixing red alder indirectly accelerates ecosystem nitrogen cycling. Ecosystems 15:1182–93.CrossRefGoogle Scholar
  51. Peterjohn WT, Correll DL. 1984. Nutrient dynamics in an agricultural watershed: observations on the role of a riparian forest. Ecology 65:1466–75.CrossRefGoogle Scholar
  52. Petrone K, Buffam I, Laudon H. 2007. Hydrologic and biotic control of nitrogen export during snowmelt: a combined conservative and reactive tracer approach. Water Resour Res 43:W06420. doi: 10.1029/2006WR005286.CrossRefGoogle Scholar
  53. Phillips RP, Brzostek E, Midgley MG. 2013. The mycorrhizal-associated nutrient economy: a new framework for predicting carbon—nutrient couplings in temperate forests. New Phytol 199:41–51.CrossRefPubMedGoogle Scholar
  54. Pierce DW, Barnett TP, Hidalgo HG, Das T, Bonfils C, Santer BD, Bala G, Dettinger MD, Cayan DR, Mirin A, Wood AW, Nozawa T. 2008. Attribution of declining Western U.S. Snowpack to human effects. J Clim 21:6425–44.CrossRefGoogle Scholar
  55. R Core Team. 2015. R: a language and environment for statistical computing. Vienna, Austria.
  56. Read DJ, Perez-Moreno J. 2003. Mycorrhizas and nutrient cycling in ecosystems—A journey towards relevance? New Phytol 157:475–92.CrossRefGoogle Scholar
  57. Schlesinger WH. 2009. On the fate of anthropogenic nitrogen. Proc Natl Acad Sci USA 106:203–8.CrossRefPubMedGoogle Scholar
  58. Sickman JO, Leydecker A, Chang CCY, Kendall C, Melack JM, Lucero DM, Schimel J. 2003. Mechanisms underlying export of N from high-elevation catchments during seasonal transitions. Biogeochemistry 64:1–24.CrossRefGoogle Scholar
  59. Stark JM, Firestone MK. 1995. Mechanisms for soil-moisture effects on activity of nitrifying bacteria. Appl Environ Microbiol 61:218–21.PubMedPubMedCentralGoogle Scholar
  60. Stark JM, Firestone MK. 1996. Kinetic characteristics of ammonium-oxidizer communities in a California oak woodland-annual grassland. Soil Biol Biochem 28:1307–17.CrossRefGoogle Scholar
  61. Tai X, Mackay DS, Anderegg WRL, Sperry JS, Brooks PD. 2016. Plant hydraulics improves and topography mediates prediction of aspen mortality in southwestern USA. New Phytol. doi: 10.1111/nph.14098.PubMedGoogle Scholar
  62. Taylor PG, Townsend AR. 2010. Stoichiometric control of organic carbon-nitrate relationships from soils to the sea. Nature 464:1178–81.CrossRefPubMedGoogle Scholar
  63. Templer P, Mack M, Chapin FI, Christenson L, Compton J, Crook H, Currie W, Curtis C, Dail D, D’Antonio C, Emmett B, Epstein H, Goodale C, Gundersen P, Hobbie S, Holland K, Hooper D, Hungate B, Lamontagne S, Nadelhoffer K, Osenberg C, Perakis S, Schleppi P, Schimel J, Schmidt I, Sommerkorn M, Spoelstra J, Tietema A, Wessel W, Zak D. 2012. Sinks for nitrogen inputs in terrestrial ecosystems: a meta-analysis of 15N tracer field studies. Ecology 93:1816–29.CrossRefPubMedGoogle Scholar
  64. Thomas RQ, Canham CD, Weathers KC, Goodale CL. 2009. Increased tree carbon storage in response to nitrogen deposition in the US. Nat Geosci 3:13–7. doi: 10.1038/ngeo721.CrossRefGoogle Scholar
  65. Thompson SE, Harman CJ, Troch PA, Brooks PD, Sivapalan M. 2011. Spatial scale dependence of ecohydrologically mediated water balance partitioning: a synthesis framework for catchment ecohydrology. Water Resour Res 47:W00J03. doi: 10.1029/2010WR009998.Google Scholar
  66. Turner MG, Smithwick EA, Metzger KL, Tinker DB, Romme WH. 2007. Inorganic nitrogen availability after severe stand-replacing fire in the greater yellowstone ecosystem. Proc Natl Acad Sci USA 104:4782–9.CrossRefPubMedPubMedCentralGoogle Scholar
  67. van Verseveld WJ, McDonnell JJ, Lajtha K. 2009. The role of hillslope hydrology in controlling nutrient loss. J Hydrol 367:177–87.CrossRefGoogle Scholar
  68. Vázquez-Ortega A, Huckle D, Perdrial J, Amistadi MK, Durcik M, Rasmussen C, McIntosh J, Chorover J. 2016. Solid-phase redistribution of rare earth elements in hillslope pedons subjected to different hydrologic fluxes. Chem Geol 426:1–18.CrossRefGoogle Scholar
  69. Vinton MA, Goergen EM. 2006. Plant-soil feedbacks contribute to the persistence of Bromus inermis in tallgrass prairie. Ecosystems 9:967–76.CrossRefGoogle Scholar
  70. Vitousek PM, Gosz JR, Grier CC, Mellilo JM, Reiners WA, Todd RL. 1979. Nitrate losses from disturbed ecosystems. Science 204:469–74.CrossRefPubMedGoogle Scholar
  71. Wexler SK, Goodale CL, McGuire KJ, Bailey SW, Groffman PM. 2014. Isotopic signals of summer denitrification in a northern hardwood forested catchment. Proc Natl Acad Sci USA 111:16413–18.CrossRefPubMedPubMedCentralGoogle Scholar
  72. Yanai RD, Vadeboncoeur MA, Hamburg SP, Arthur MA, Fuss CB, Groffman PM, Siccama TG, Driscoll CT. 2013. From missing source to missing sink: long-term changes in the nitrogen budget of a northern hardwood forest. Environ Sci Technol 47:11440–8.CrossRefPubMedPubMedCentralGoogle Scholar
  73. Yuan F, Arain MA, Black TA, Morgenstern K. 2007. Energy and water exchanges modulated by soil-plant nitrogen cycling in a temperate Pacific Northwest conifer forest. Ecol Model 201:331–47.CrossRefGoogle Scholar
  74. Zak D, Groffman P, Pregitzer K, Christensen S, Tiedje J. 1990. The vernal dam: plant-microbe competition for nitrogen in northern hardwood forests. Ecology 71:651–6.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Samantha R. Weintraub
    • 1
    • 2
    Email author
  • Paul D. Brooks
    • 1
    • 2
  • Gabriel J. Bowen
    • 1
    • 2
  1. 1.Department of Geology and GeophysicsUniversity of UtahSalt Lake CityUSA
  2. 2.Global Change and Sustainability CenterUniversity of UtahSalt Lake CityUSA

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