Linking Above- and Belowground Responses to 16 Years of Fertilization, Mowing, and Removal of the Dominant Species in a Temperate Grassland
Species-rich oligotrophic meadows are affected by a wide range of management interventions that influence their functioning and capacity to deliver ecosystem services, but long-term studies on the above- and belowground adaptations to different management tools are still scarce. We focused on the interactive effects of NPK fertilization, mowing, and removal of the initially dominant species (Molinia caerulea) on plant, soil, and microbial responses in wet oligotrophic grassland in a 16-year full-factorial manipulative experiment. Changes in vegetation composition, soil pH, and nutrient availability were accompanied by altered microbial phospholipid fatty acid (PLFA) composition, whereas treatment effects on soil microbial biomass and carbon (C) mineralization were mainly related to changes in soil organic matter (SOM) content and nutrient availability. Fertilization decreased plant species richness aboveground and lowered SOM storage and microbial activity belowground. Mowing preserved high plant diversity and led to more efficient recycling of N within the grassland, whereas Molinia removal significantly affected only plant community composition. Mowing combined with fertilization maintained high species richness only in the short term. Belowground, mowing reduced N leaching from the fertilized system but did not prevent SOM depletion, soil acidification, and concomitant adverse effects on soil microbes. We conclude that annual mowing is the appropriate type of extensive management for oligotrophic species-rich meadows, but the concomitant nutrient depletion should not be compensated for by regular NPK fertilization due to its adverse effects on soil quality.
Keywordsmicrobial community structure PLFA grassland mowing fertilization dominant removal pH
The research was supported by the Grant Agency of the Czech Republic (GAČR, Project No. 13-17118S). We thank Gerhard Kerstiens for his help with the language.
- Chytrý M. 2012. Vegetation of the Czech Republic: diversity, ecology, history and dynamics. Preslia 84:427–504.Google Scholar
- Hedlund K, Regina IS, Van der Putten WH, Lepš J, Díaz T, Korthals GW, Lavorel S, Brown VK, Gormsen D, Mortimer SR, Barrueco CR, Roy J, Smilauer P, Smilauerova M, Van Dijk C. 2003. Plant species diversity, plant biomass and responses of the soil community on abandoned land across Europe: idiosyncracy or above-belowground time lags. Oikos 103:45–58.CrossRefGoogle Scholar
- Hejcman M, Sochorova L, Pavlu V, Strobach J, Diepolder M, Schellberg J. 2014. The Steinach grassland experiment: soil chemical properties, sward height and plant species composition in three cut alluvial meadow after decades-long fertilizer application. Agric Ecosyst Environ 184:76–87.CrossRefGoogle Scholar
- Kaiser Ch, Koranda M, Kitzler B, Fuchslueger L, Schnecker J, Schweiger P, Rasche F, Zechmeister-Boltenstern S, Sessitch A, Richter A. 2010. Belowground carbon allocation by trees drives seasonal patterns of extracellular enzyme activities by altering microbial community composition in a beech forest soil. New Phytol 187:843–58.CrossRefPubMedPubMedCentralGoogle Scholar
- Kroppenstedt RM. 1985. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Minikin DE, Eds. Chemical methods in bacterial systematics. London: Academic Press. p 173–94.Google Scholar
- Lange M, Eisenhauer N, Sierra CA, Bessler H, Engels C, Griffiths RI, Mellado-Vázquez PG, Malik AA, Roy J, Scheu S, Steinbeiss S, Thomson BC, Trumbore SE, Gleixner G. 2015. Plant diversity increases soil microbial activity and soil carbon storage. Nat Commun 6:6707. doi: 10.1038/ncomms7707.CrossRefPubMedGoogle Scholar
- Legay N, Grassein F, Binet MN, Arnoldi C, Personeni E, Perigon S, Poly F, Pommier T, Puissant J, Clément JC, Lavorel S, Mouhamadou B. 2016. Plant species identities and fertilization influence on arbuscular mycorrhizal fungal colonization and soil bacterial activities. Appl Soil Ecol 98:132–9.CrossRefGoogle Scholar
- Nyborg M, Molina-Ayala M, Solberg ED, Izaurralde EC, Malhi SS, Janzen HH. 1997. Carbon storage in grassland soils as related to N and S fertilizers. In: Lal R, Ed. Management of carbon sequestration in soil. Boca Raton: CRC Press. p 421–32.Google Scholar
- Ter Braak CJF, Šmilauer P. 2012. Canoco reference manual and user’s guide: software for ordination, version 5.0. Ithaca: Microcomputer Power. p 496.Google Scholar
- Thomas GW. 1982. Exchangeable cations. In: Page AL, Ed. Methods of soil analysis, Part 2 Chemical and microbiological properties, 2nd edition. Agronomy 9. pp 159–65.Google Scholar
- Tóth G. 2008. Soil quality in the European Union. In: Tóth G, Montanarella L, Rusco E, Eds. Threats to soil quality in Europe. JRC Scientific and Technical Reports. pp 11–9.Google Scholar