, Volume 20, Issue 2, pp 354–367 | Cite as

Linking Above- and Belowground Responses to 16 Years of Fertilization, Mowing, and Removal of the Dominant Species in a Temperate Grassland

  • Petr Kotas
  • Michal Choma
  • Hana Šantrůčková
  • Jan Lepš
  • Jan Tříska
  • Eva Kaštovská


Species-rich oligotrophic meadows are affected by a wide range of management interventions that influence their functioning and capacity to deliver ecosystem services, but long-term studies on the above- and belowground adaptations to different management tools are still scarce. We focused on the interactive effects of NPK fertilization, mowing, and removal of the initially dominant species (Molinia caerulea) on plant, soil, and microbial responses in wet oligotrophic grassland in a 16-year full-factorial manipulative experiment. Changes in vegetation composition, soil pH, and nutrient availability were accompanied by altered microbial phospholipid fatty acid (PLFA) composition, whereas treatment effects on soil microbial biomass and carbon (C) mineralization were mainly related to changes in soil organic matter (SOM) content and nutrient availability. Fertilization decreased plant species richness aboveground and lowered SOM storage and microbial activity belowground. Mowing preserved high plant diversity and led to more efficient recycling of N within the grassland, whereas Molinia removal significantly affected only plant community composition. Mowing combined with fertilization maintained high species richness only in the short term. Belowground, mowing reduced N leaching from the fertilized system but did not prevent SOM depletion, soil acidification, and concomitant adverse effects on soil microbes. We conclude that annual mowing is the appropriate type of extensive management for oligotrophic species-rich meadows, but the concomitant nutrient depletion should not be compensated for by regular NPK fertilization due to its adverse effects on soil quality.


microbial community structure PLFA grassland mowing fertilization dominant removal pH 



The research was supported by the Grant Agency of the Czech Republic (GAČR, Project No. 13-17118S). We thank Gerhard Kerstiens for his help with the language.

Supplementary material

10021_2016_31_MOESM1_ESM.pdf (362 kb)
Supplementary material 1 (PDF 362 kb)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Petr Kotas
    • 1
    • 2
  • Michal Choma
    • 1
  • Hana Šantrůčková
    • 1
  • Jan Lepš
    • 3
    • 4
  • Jan Tříska
    • 2
  • Eva Kaštovská
    • 1
  1. 1.Department of Ecosystem Biology, Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  2. 2.Laboratory of Metabolomics and Isotopic Analyses, Global Change Research InstituteAcademy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  3. 3.Department of Botany, Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  4. 4.Institute of EntomologyAcademy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic

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