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Ecosystems

, Volume 18, Issue 5, pp 867–880 | Cite as

Soil Microbes Compete Strongly with Plants for Soil Inorganic and Amino Acid Nitrogen in a Semiarid Grassland Exposed to Elevated CO2 and Warming

  • Janet Chen
  • Yolima Carrillo
  • Elise Pendall
  • Feike A. Dijkstra
  • R. Dave Evans
  • Jack A. Morgan
  • David G. Williams
Article

Abstract

Free amino acids (FAAs) in soil are an important N source for plants, and abundances are predicted to shift under altered atmospheric conditions such as elevated CO2. Composition, plant uptake capacity, and plant and microbial use of FAAs relative to inorganic N forms were investigated in a temperate semiarid grassland exposed to experimental warming and free-air CO2 enrichment. FAA uptake by two dominant grassland plants, Bouteloua gracilis and Artemesia frigida, was determined in hydroponic culture. B. gracilis and microbial N preferences were then investigated in experimental field plots using isotopically labeled FAA and inorganic N sources. Alanine and phenylalanine concentrations were the highest in the field, and B. gracilis and A. frigida rapidly consumed these FAAs in hydroponic experiments. However, B. gracilis assimilated little isotopically labeled alanine, ammonium and nitrate in the field. Rather, soil microbes immobilized the majority of all three N forms. Elevated CO2 and warming did not affect plant or microbial uptake. FAAs are not direct sources of N for B. gracilis, and soil microbes outcompete this grass for organic and inorganic N when N is at peak demand within temperate semiarid grasslands.

Keywords

amino acids global change grasslands nitrogen uptake 1315

Notes

Acknowledgments

Special thanks to David Legg, David Perry, Dan Lecain, Chandelle MacDonald, Amanda Brennan, Dana Blumenthal, Justin Jones, Erik Hobbie, and Jane Zelikova for manuscript, laboratory, and field assistance. This material is based upon work supported by the US Department of Agriculture Agricultural Research Service Climate Change, Soils & Emissions Program, USDA-CSREES Soil Processes Program (#2008-35107-18655), US Department of Energy Office of Science (BER), through the Terrestrial Ecosystem Science program (#DE-SC0006973) and the Western Regional Center of the National Institute for Climatic Change Research, and by the National Science Foundation (DEB#1021559). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Janet Chen
    • 1
  • Yolima Carrillo
    • 2
  • Elise Pendall
    • 2
    • 3
  • Feike A. Dijkstra
    • 4
  • R. Dave Evans
    • 5
  • Jack A. Morgan
    • 6
  • David G. Williams
    • 3
  1. 1.Department of Ecosystem Science and Management & Program in EcologyUniversity of WyomingLaramieUSA
  2. 2.Hawkesbury Institute for the EnvironmentUniversity of Western SydneyPenrithAustralia
  3. 3.Department of Botany and Program in EcologyUniversity of WyomingLaramieUSA
  4. 4.Department of Environmental Sciences, Centre for Carbon, Water and FoodThe University of SydneyCamdenAustralia
  5. 5.Rangeland Resources Research UnitUSDA Agricultural Research ServiceFort CollinsUSA
  6. 6.School of Biological Sciences, WSU Stable Isotope Core FacilityWashington State UniversityPullmanUSA

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