Simple action potential measurement of cardiac cell sheet utilizing electronic sheet

  • Takashi Ohya
  • Kazuki Nakazono
  • Tetsutaro Kikuchi
  • Daisuke Sasaki
  • Katsuhisa Sakaguchi
  • Tatsuya Shimizu
  • Kenjiro Fukuda
  • Takao Someya
  • Shinjiro Umezu
Original Article
  • 35 Downloads

Abstract

In this study, a device for measuring the action potential of cardiac cell sheets was developed. The action potential was measured using a device comprising a 2-µm-thick parylene film with a silver electrode printed on it, which was referred to as the “electronic sheet.” The thin parylene film exhibits high biocompatibility and flexibility. Therefore, it demonstrates promise for biomedical microelectromechanical system applications. In this study, a cell sheet was used because the interest it had garnered in regenerative medicine for creating cardiac tissue in vitro similar to that in vivo. A high-efficiency drug development system can be realized by combining cell sheet technology and fabricating a flexible electronic sheet. The action potential of a cardiac cell sheet from a rat was measured using the as-developed flexible electronic sheet.

Keywords

Regenerative medicine Printed electronics Cell sheet Action potential Flexible electronics 

Notes

Acknowledgements

T. Shimizu is a member of Scientific Advisory Board of CellSeed Inc. and is a shareholder of CellSeed Inc. CellSeed Inc. has licenses for certain cell sheet-related technologies and patents from Tokyo Women’s Medical University. Tokyo Women’s Medical University received research funds from CellSeed Inc. This work was supported by JSPS Kakenhi Grant number 16H04308 and 16K14203.

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Copyright information

© ISAROB 2018

Authors and Affiliations

  • Takashi Ohya
    • 1
    • 2
  • Kazuki Nakazono
    • 1
    • 2
  • Tetsutaro Kikuchi
    • 2
  • Daisuke Sasaki
    • 2
  • Katsuhisa Sakaguchi
    • 3
  • Tatsuya Shimizu
    • 2
  • Kenjiro Fukuda
    • 4
    • 5
  • Takao Someya
    • 4
  • Shinjiro Umezu
    • 6
  1. 1.Graduate School of Creative Science and EngineeringWaseda UniversityTokyoJapan
  2. 2.Institute of Advanced Biomedical Engineering and Science, TWInsTokyo Women’s Medical UniversityTokyoJapan
  3. 3.Faculty of Science and Engineering, TWInsWaseda UniversityTokyoJapan
  4. 4.RIKEN Thins-Film Device Laboratory and Center for Emergent Mater ScienceSaitamaJapan
  5. 5.JST PRESTOSaitamaJapan
  6. 6.Undergraduate School of Creative Science and EngineeringWaseda UniversityTokyoJapan

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