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Brain Tumor Pathology

, Volume 33, Issue 3, pp 222–227 | Cite as

High incidence of TERT mutation in brain tumor cell lines

  • Tanner M. Johanns
  • Yujie Fu
  • Dale K. Kobayashi
  • Yu Mei
  • Ian F. Dunn
  • Diane D. Mao
  • Albert H. Kim
  • Gavin P. Dunn
Rapid Short communication

Abstract

TERT promoter gene mutations are highly recurrent in malignant glioma. However, little information exists regarding their presence in experimental brain tumor models. To better characterize systems in which TERT mutation studies could be appropriately modeled experimentally, the TERT promoter was examined by conventional sequencing in primary brain tumor initiating cells (BTIC), two matched recurrent BTIC lines, a panel of established malignant glioma cell lines, and two meningioma cell lines. Telomerase gene expression was examined by quantitative PCR. We found that all glioblastoma BTIC lines harbored a TERT mutation, which was retained in two patient-matched recurrent BTIC. The TERT C228T or C250T mutation was found in 33/35 (94 %) of established malignant glioma cell lines and both meningioma cell lines examined. Brain tumor cell lines expressed variably high telomerase levels. Thus, a high percentage of glioma cell lines, as well as two meningioma cell lines, harbors TERT mutations. These data characterize tractable, accessible models with which to further explore telomerase biology in these tumor types.

Keywords

TERT Telomerase Glioma Glioblastoma Meningioma Brain tumor initiating cells Cancer 

Notes

Acknowledgements

The authors thank Dr. Ravi Uppaluri for reviewing this manuscript and Dr. Jeffrey Atkinson for technical assistance. This work was supported by NIH grant K08NS092912 (G.P.D.), NIH Grant K08NS081105 (A.H.K.), American Cancer Society-Institutional Research Grant (A.H.K., G.P.D), the Duesenberg Research Fund (A.H.K.), the Physician-Scientist Training Program at Washington University School of Medicine (T.M.J.), and the Brain Science Foundation (I.F.D.).

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Copyright information

© The Japan Society of Brain Tumor Pathology 2016

Authors and Affiliations

  1. 1.Division of Oncology, Department of MedicineWashington University School of MedicineSt. LouisUSA
  2. 2.Center for Human Immunology and Immunotherapy ProgramsWashington University School of MedicineSt. LouisUSA
  3. 3.Department of Neurological Surgery, Center for Human Immunology and Immunotherapy ProgramsWashington University School of MedicineSt. LouisUSA
  4. 4.Department of NeurosurgeryBrigham and Women’s HospitalBostonUSA
  5. 5.The Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of MedicineSt. LouisUSA

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