Advertisement

Brain Tumor Pathology

, Volume 27, Issue 1, pp 17–21 | Cite as

Nestin expression in astrocytic tumors delineates tumor infiltration

  • Ryuhei KitaiEmail author
  • Ryosuke Horita
  • Kazufumi Sato
  • Kazuhiko Yoshida
  • Hidetaka Arishima
  • Yoshifumi Higashino
  • Norichika Hashimoto
  • Hiroaki Takeuchi
  • Toshihiko Kubota
  • Ken-Ichiro Kikuta
Original Article

Abstract

Nestin is an intermediate filament protein expressed in undifferentiated cells during central nervous system development, and glioma is known to be a highly infiltrative tumor. We determined whether nestin was expressed in astrocytic tumors and could identify infiltrating tumor cells. We screened 65 archival, paraffin-embedded adult astrocytic tumors using immunohistochemical staining and computerized overlaid photographs. Normal biopsied brains and metastatic brain tumors were also examined. The intensity of nestin expression corresponded to the tumor grade. All 33 glioblastoma cases showed positive and extensive staining, which was less positive in diffuse astrocytoma. Overlaid images showed that nestin immunostaining delineated tumor invasion into adjacent gray and white matter. Nestin is a useful marker for examining the infiltration of malignant cells into surrounding tissue.

Key words

Nestin Glioma Invasion 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Duggal N, Hammond RR (2002) Nestin expression in ganglioglioma. Exp Neurol 174:89–95CrossRefPubMedGoogle Scholar
  2. 2.
    Hockfield S, McKay RDG (1985) Identification of major cell classes in the developing mammalian nervous system. J Neurosci 5:3310–3328PubMedGoogle Scholar
  3. 3.
    Tohyama T, Lee VM-Y, Rorke LB, et al (1992) Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells. Lab Invest 66:303–313PubMedGoogle Scholar
  4. 4.
    Dahlstrand J, Collins VP, Lendahl U (1992) Expression of the class VI intermediate filament nestin in human central nervous system tumors. Cancer Res 52:5334–5341PubMedGoogle Scholar
  5. 5.
    Florenes VA, Holm R, Myklebost O, et al (1994) Expression of the neuroectodermal intermediate filament nestin in human melanomas. Cancer Res 54:354–356PubMedGoogle Scholar
  6. 6.
    Kobayashi M, Sjoberg G, Soderhall S, et al (1998) Pediatric rhabdomyosarcomas express the intermediate filament nestin. Pediatr Res 43:386–392CrossRefPubMedGoogle Scholar
  7. 7.
    Almqvist PM, Mah R, Lendahl U, et al (2002) Immunohistochemical detection of nestin in pediatric brain tumors. J Histochem Cytochem 50:147–158PubMedGoogle Scholar
  8. 8.
    Duggal N, Iskander S, Hammond RR (2003) MAP2 and nestin co-expression in dysembryoplastic neuroepithelial tumors. Clin Neuropathol 22:57–65PubMedGoogle Scholar
  9. 9.
    Louis DN, Ohgaki H, Wiestler OD, et al (2007) WHO classification of tumours of the central nervous system. Pathology and genetics of tumors of the nervous system. International Agency for Research on Cancer, World Health Organization, Lyon, pp 13-52Google Scholar
  10. 10.
    Takano T, Becker LE (1997) Developmental change of the nestinimmunoreactive midline raphe glial structure in human brainstem and spinal cord. Dev Neurosci 19:202–209CrossRefPubMedGoogle Scholar
  11. 11.
    Johansson CB, Momma S, Clarke DL, et al (1999) Identification of a neural stem cell in the adult mammalian central nervous system. Cell 96:25–34CrossRefPubMedGoogle Scholar
  12. 12.
    Frisen J, Johansson CB, Torok C, et al (1995) Rapid, widespread, and long-lasting induction of nestin contributes to the generation of glial scar tissue after CNS injury. J Cell Biol 131:453–464CrossRefPubMedGoogle Scholar
  13. 13.
    Krum JM, Rosenstein JM (1999) Transient coexpression of nestin, GFAP, and vascular endothelial growth factor in mature reactive astroglia following neural grafting or brain wounds. Exp Neurol 160:348–360CrossRefPubMedGoogle Scholar
  14. 14.
    Li Y, Chopp M (1999) Temporal profile of nestin expression after focal cerebral ischemia in adult rat. Brain Res 838:1–10CrossRefPubMedGoogle Scholar
  15. 15.
    Lin RC, Matesic DF, Marvin M, et al (1995) Re-expression of the intermediate filament nestin in reactive astrocytes. Neurobiol Dis 2:79–85CrossRefPubMedGoogle Scholar
  16. 16.
    Ehrmann J, Kolar Z, Mokry J (2005) Nestin as a diagnostic and prognostic marker: immunohistochemical analysis of its expression in different tumors. J Clin Pathol 58:222–223CrossRefPubMedGoogle Scholar
  17. 17.
    Sugawara K, Kurihara H, Negishi M, et al (2002) Nestin as a marker for proliferative endothelium in gliomas. Lab Invest 82:345–351PubMedGoogle Scholar
  18. 18.
    Suzuki SO, Kitai R, Llena J, et al (2002) MAP-2e, a novel MAP-2 isoform, is expressed in gliomas and delineates tumor architecture and patterns of infiltration. J Neuropathol Exp Neurol 61:403–412PubMedGoogle Scholar

Copyright information

© Japan Soceity of BrainTumor Pathology 2010

Authors and Affiliations

  • Ryuhei Kitai
    • 1
    Email author
  • Ryosuke Horita
    • 1
  • Kazufumi Sato
    • 1
  • Kazuhiko Yoshida
    • 1
  • Hidetaka Arishima
    • 1
  • Yoshifumi Higashino
    • 1
  • Norichika Hashimoto
    • 1
  • Hiroaki Takeuchi
    • 1
  • Toshihiko Kubota
    • 1
  • Ken-Ichiro Kikuta
    • 1
  1. 1.Department of Neurosurgery, Faculty of Medical SciencesFukui UniversityFukuiJapan

Personalised recommendations