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Journal of Solid State Electrochemistry

, Volume 23, Issue 12, pp 3391–3398 | Cite as

A sensitive electrochemical aptasensor based on MB-anchored GO for the rapid detection of Cronobacter sakazakii

  • Haixia Peng
  • Yuanyuan Hui
  • Rong Ren
  • Bini WangEmail author
  • Shuanghong Song
  • Yaping He
  • Fuxin Zhang
Original Paper
  • 24 Downloads

Abstract

Cronobacter sakazakii (C. sakazakii) can cause extremely high mortality diseases especially in infants, so it is necessary to rapidly and specifically detect C. sakazakii in powdered infant formula (PIF). In this work, the amplification strategy of methylene blue (MB)-anchored graphene oxide (GO) was used to develop an electrochemical aptasensor for detecting C. sakazakii easily and quickly. In the absence of C. sakazakii, GO would be bound to the aptamer of C. sakazakii by π-π interaction, and then adsorbed MB through electrostatic adherence; thus, the electrochemical signal of MB amplified. While in the presence of C. sakazakii, the bacteria would be specifically combined with the aptamer, and hindered the electrostatic adsorption of GO and MB to the aptamer, resulting in the decreasing electrochemical signal of MB. The fabricated electrochemical aptasensor showed a wide linear range from 2 × 101 to 2 × 106 CFU mL−1 of the bacteria concentration, and its limit of detection reached 7 CFU mL−1 (S/N = 3). In addition, the applicability of the aptasensor in PIF for the analysis of C. sakazakii was successfully demonstrated. This aptasensor would provide a new method for the detection of C. sakazakii but also a novel sensing platform for the rapid detection of pathogen bacteria.

Keywords

C. sakazakii Graphene oxide Aptamer Electrochemical biosensor 

Notes

Funding

This research was funded by the Key R&D Program Projects in Shaanxi Province (No.2019ZDLNY06-06) and the Fundamental Research Funds for the Central Universities (No. GK201803077 and GK201806008).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Food Engineering and Nutritional ScienceShaanxi Normal UniversityXi’anChina
  2. 2.Shaanxi Provincial Key Laboratory of Electroanalytical ChemistryNorthwest UniversityXi’anChina
  3. 3.Key Laboratory of Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life SciencesShaanxi Normal UniversityXi’anChina
  4. 4.School of Chemical EngineeringXi’an UniversityXi’anChina

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