Journal of Solid State Electrochemistry

, Volume 22, Issue 1, pp 169–180 | Cite as

Coupled electrochemical-chemical procedure used in construction of molecularly imprinted polymer-based electrode: a highly sensitive impedimetric melamine sensor

  • Mojtaba Shamsipur
  • Nozar Moradi
  • Afshin Pashabadi
Original Paper
  • 96 Downloads

Abstract

A novel molecularly imprinted sensor was fabricated and used for the impedimetric detection of melamine. Considering the identity of polymeric film and the pK a of a melamine template, an effective procedure was established to construct the MIP-based melamine sensor. The proposed method is based on the electropolymerization of pyrrole (Py) in the presence of melamine on the electrochemically reduced graphene oxide modified glassy carbon electrode (ERGO/GCE), followed by treatment with the solution of 1% H2O2 in alkaline water/CH3CN-mixed solvents. The surface morphology and the electrical feature of molecularly imprinted polymer (MIP) were characterized by scanning electron microscopy (SEM), Fourier transformation infrared spectroscopy (FTIR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The EIS was also utilized to transduce the change of charge transfer resistance (R ct) at the interface of polymer film-electrolyte, after subsequent incubation of electrode in the solution containing different concentrations of analyte, and consequently, a linear response was obtained over the range of 4.0 to 240 nM with a detection limit of 0.83 nM (S/N = 3). The effect of possible interferences on the response of sensor was studied, and the results confirmed the good selectivity of the proposed device for melamine assay. The MIP sensor was successfully applied to determine melamine in a multiple concentration-spiked milk sample.

Keywords

Impedimetric sensor Molecularly imprinted polymer Electropolymerization Melamine Pyrrole 

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of ChemistryRazi UniversityKermanshahIran

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