Journal of Solid State Electrochemistry

, Volume 19, Issue 7, pp 2189–2197 | Cite as

DNA-binding studies of complex of Pt(bpy)(pip)]2+ and [Pt(bpy)(hpip)]2+ by electrochemical methods: development of an electrochemical DNA biosensor

  • Izzet Kocak
  • Ufuk Yildiz
  • Burak Coban
  • Abdurrahman Sengul
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

The electrochemical properties and deoxyribonucleic acid (DNA)-binding affinities of [Pt(bpy)(pip)]2+ (1) and [Pt(bpy)(hpip)]2+ (2) in homogeneous solution were studied. These platinum(II) complexes have shown to interact with DNA via intercalation mode. For the cyclic voltammetry (CV) experiments, since 2 leads to a larger decrease in the peak current and more positive shift in the peak potential in comparison to the analogous compound of 1, it could be noted that 2 exhibits higher intercalative binding affinity against DNA. The effect of ionic strength and competitive binding studies in the presence of ethidium bromide (EB) were also investigated by CV. Electrochemical DNA biosensor based on the immobilization of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) probe and 1 and 2 onto electrochemically activated glassy carbon (GC) electrode was also achieved. The immobilization of dsDNA and ssDNA probe and hybridization were monitored by differential pulse voltammetry using 1 and 2 as hybridization indicators. It was found that both platinum complexes show larger and more evident electrochemical signals for the hybridized probe dsDNA with respect to ssDNA immobilized on GC electrode. The developed electrochemical DNA biosensor showed good selectivity and analytical performance for the complementary target nucleotide with limit of detection of 1.23 × 10−8 and 8.05 × 10−9 mol L−1 and limit of quantification of 4.06 × 10−7 and 2.66 × 10−8 for 1 and 2, respectively.

Keywords

Glassy Carbon Glassy Carbon Electrode Ethidium Bromide Differential Pulse Voltammetry Platinum Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This work is funded by The Scientific and Technological Research Council of Turkey. Authors also thank Assistant Professor M. Emre HANHAN for his research facilities.

Supplementary material

10008_2015_2859_MOESM1_ESM.docx (314 kb)
ESM 1 (DOCX 314 kb)

References

  1. 1.
    Gao F, Wang Q, Zheng M, Li S, Chen G, Jiao K (2011) Int J Electrochem Sci 6:1508–1521Google Scholar
  2. 2.
    Li G, Liu N, Liu S, Zhang S (2008) Electrochim Acta 53:2870–2876CrossRefGoogle Scholar
  3. 3.
    Palecek E, Fojta M (2001) Anal Chem 73:73A–83AGoogle Scholar
  4. 4.
    Arshad N, Farooqi SI, Bhatti MH, Saleem S, Mirza B (2013) J Photochem Photobiol B 125:70–82CrossRefGoogle Scholar
  5. 5.
    Wang Q, Wang X, Yu Z, Yuan X, Jiao K (2011) Int J Electrochem Sci 6:5470–5481Google Scholar
  6. 6.
    Kelly J, Tossi A, McConnell D, Uigin O (1985) Nucleic Acids Res 13:6017–6034CrossRefGoogle Scholar
  7. 7.
    Li F, Chen W, Tang C, Zhang S (2008) Talanta 77:1–8CrossRefGoogle Scholar
  8. 8.
    Maheswari PU, Rajendiran V, Stoeckli-Evans H, Palaniandavar M (2006) Inorg Chem 45:37–50CrossRefGoogle Scholar
  9. 9.
    Gaudry E, Aubard J, Amouri H, Lévi G, Cordier C (2006) Biopolymers 82:399–404CrossRefGoogle Scholar
  10. 10.
    Zhou CY, Zhao J, Wu YB, Yin CX, Pin Y (2007) J Inorg Biochem 101:10–18CrossRefGoogle Scholar
  11. 11.
    Xu G, Fan J, Jiao K (2008) Electroanalysis 20:1209–1214CrossRefGoogle Scholar
  12. 12.
    Niu S, Li F, Zhang S, Wang L, Li X, Wang S (2006) Sensors 6:1234–1244CrossRefGoogle Scholar
  13. 13.
    Del Pozo MV, Alonso C, Pariente F, Lorenzo E (2005) Biosens Bioelectron 20:1549–1558CrossRefGoogle Scholar
  14. 14.
    Del Pozo MV, Alonso C, Pariente F, Lorenzo E (2005) Anal Chem 77:2550–2557CrossRefGoogle Scholar
  15. 15.
    Guin PS, Das P, Das S, Mandal PC (2012) Int J Electrochem 2012:1–10CrossRefGoogle Scholar
  16. 16.
    Wang QX, Jiao K, Sun W, Jian FF, Hu X (2006) Eur J Inorg Chem 2006:1838–1845CrossRefGoogle Scholar
  17. 17.
    Daniel S, Rao TP, Rao KS, Rani SU, Naidu GRK, Lee HY, Kawai T (2007) Sensors Actuators B Chem 122:672–682CrossRefGoogle Scholar
  18. 18.
    Wang L, Wang X, Chen X, Liu J, Liu S, Zhao C (2012) Bioelectrochemistry 88:30–35CrossRefGoogle Scholar
  19. 19.
    Guo M, Chen J, Liu D, Nie L, Yao S (2004) Bioelectrochemistry 62:29–35CrossRefGoogle Scholar
  20. 20.
    Rafique B, Khalid AM, Akhtar K, Jabbar A (2013) Biosens Bioelectron 44:21–26CrossRefGoogle Scholar
  21. 21.
    Wang SM, Su WY, Cheng SH (2010) Int J Electrochem Sci 5:1649Google Scholar
  22. 22.
    Li XM, Ju HQ, Ding CF, Zhang SS (2007) Anal Chim Acta 582:158–163CrossRefGoogle Scholar
  23. 23.
    Coban B, Yildiz U (2014) Appl Biochem Biotechnol 172:248–262CrossRefGoogle Scholar
  24. 24.
    Chen H, Dou C, Wu Y, Li H, Xi X, Yang P (2009) J Inorg Biochem 103:827–832CrossRefGoogle Scholar
  25. 25.
    Maheswari PU, Palaniandavar MJ (2004) J Inorg Biochem 98:219–230CrossRefGoogle Scholar
  26. 26.
    Zhang QL, Liu JG, Liu J, Xue GQ, Li H, Liu JZ, Zhou H, Qu LH, Ji LN (2001) J Inorg Biochem 85:291–296CrossRefGoogle Scholar
  27. 27.
    Coban B, Yildiz U, Şengul A (2013) J Biol Inorg Chem 18:461–471CrossRefGoogle Scholar
  28. 28.
    Cusumano M, Di Pietro ML, Giannetto A (2006) Inorg Chem 45:230–235CrossRefGoogle Scholar
  29. 29.
    Niu SY, Zhang SS, Wang L, Li XM (2006) J Electroanal Chem 597:111–118CrossRefGoogle Scholar
  30. 30.
    Wang J, Cai X, Rivas G, Shiraishi H, Farias PAM, Dontha N (2007) Anal Chem 68:2629–2634CrossRefGoogle Scholar
  31. 31.
    Liu X-W, Lu J-L, Chen Y-D, Li L, D-S Z (2011) Inorg Chim Acta 379:1–6CrossRefGoogle Scholar
  32. 32.
    Janjua NK, Akhter Z, Jabeen F, Iftikhar B (2014) J t Korean Chem Soc 53:153–159CrossRefGoogle Scholar
  33. 33.
    Noorbakhsh A, Salimi A (2011) Biosens Bioelectron 30:188–196CrossRefGoogle Scholar
  34. 34.
    Shujhaa S, Shaha A, Rehmana Z, Muhammada N, Alia S, Qureshia R, Khalid N, Meetsma A (2010) Eur J Med Chem 45:2902–2911CrossRefGoogle Scholar
  35. 35.
    Sirajuddin M, Ali S, Badshah A (2013) J Photochem Photobiol B 124:1–19CrossRefGoogle Scholar
  36. 36.
    Lu X, Zhang M, Kang J, Wang X, Zhuo L, Liu H (2004) J Inorg Biochem 98:582–588CrossRefGoogle Scholar
  37. 37.
    Hajian R, Shams N, Mohagheghian M (2009) J Braz Chem Soc 20:1399–1405CrossRefGoogle Scholar
  38. 38.
    Nia Y, Lina D, Kokot S (2006) Anal Biochem 352:231–242CrossRefGoogle Scholar
  39. 39.
    Bard AJ, Faulkner LR (2001) Electrochemical methods: fundamentals and applications. Wiley, New YorkGoogle Scholar
  40. 40.
    Laviron E (1979) J Electroanal Chem 101:19–28CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Izzet Kocak
    • 1
  • Ufuk Yildiz
    • 1
  • Burak Coban
    • 1
  • Abdurrahman Sengul
    • 1
  1. 1.Department of Chemistry, Faculty of Arts and SciencesBulent Ecevit UniversityZonguldakTurkey

Personalised recommendations