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Journal of Solid State Electrochemistry

, Volume 18, Issue 1, pp 207–213 | Cite as

Electrochemical myoglobin biosensor based on carbon ionic liquid electrode modified with Fe3O4@SiO2 microsphere

  • Xiaofeng Wang
  • Zheng You
  • Hailiang Sha
  • Zhaolan Sun
  • Wei SunEmail author
Original Paper

Abstract

In this paper, a Fe3O4@SiO2 core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe3O4@SiO2 microsphere could form an organic–inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe3O4@SiO2/CILE with the formal peak potential (E 0′) located at −0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe3O4@SiO2 composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe3O4@SiO2/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L−1 with the detection limit of 0.18 mmol L−1 (3σ). Based on CS/Mb-Fe3O4@SiO2/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility.

Keywords

1-Butylpyridinium hexafluorophosphate Myoglobin Direct electrochemistry Carbon ionic liquid electrode Fe3O4@SiO2 microsphere 

Notes

Acknowledgments

We are grateful for the financial support of the National Natural Science Foundation of China (nos. 30901700, 50905096).

References

  1. 1.
    Shiddiky MJA, Torriero AAJ (2011) Biosens Bioelectron 26:1775–1787CrossRefGoogle Scholar
  2. 2.
    Opallo M, Lesniewski A (2011) J Electroanal Chem 656:2–16CrossRefGoogle Scholar
  3. 3.
    Sun W, Gao RF, Jiao K (2007) Chinese J Anal Chem 35:1813–1819Google Scholar
  4. 4.
    Wang SF, Chen T, Zhang ZL, Shen XC, Lu ZX, Pang DW, Wong KY (2005) Langmuir 21:9260–9266CrossRefGoogle Scholar
  5. 5.
    Yu P, Lin YQ, Xiang L, Su L, Zhang J, Mao LQ (2005) Langmuir 21:9000–9006CrossRefGoogle Scholar
  6. 6.
    Maleki N, Safavi A, Tajabade F (2006) Anal Chem 78:3820–3826CrossRefGoogle Scholar
  7. 7.
    Sun W, Yang MX, Gao RF, Jiao K (2007) Electroanalysis 19:1597–1602CrossRefGoogle Scholar
  8. 8.
    Zhu ZH, Qu LN, Li X, Zeng Y, Sun W, Huang XT (2010) Electrochim Acta 55:5959–5965CrossRefGoogle Scholar
  9. 9.
    Gao HW, Xi MY, Qi XW, Lu M, Zhan TR, Sun W (2012) J Electroanal Chem 664:88–93CrossRefGoogle Scholar
  10. 10.
    Oktem HA, Bayramoglu G, OZalp VC, Arica MY (2007) Biotechnol Prog 23:146–154CrossRefGoogle Scholar
  11. 11.
    Hu AG, Yee GT, Lin WB (2005) J Am Chem Soc 127:12486–12487CrossRefGoogle Scholar
  12. 12.
    Kim J, Lee JE, Lee J, Yu JH, Kim BC, An K, Wang YH, Shin CH, Park JG, Kim J, Hyeon T (2006) J Am Chem Soc 128(3):688–689CrossRefGoogle Scholar
  13. 13.
    Xu XQ, Deng CH, Gao MX, Yu WJ, Yang PY, Zhang XM (2006) Adv Mater 18:3289–3293CrossRefGoogle Scholar
  14. 14.
    Li Y, Yan B, Deng CH, Yu WJ, Xu XQ, Yang PY, Zhang XM (2007) Proteomics 7:2330–2339CrossRefGoogle Scholar
  15. 15.
    Levy L, Sahoo Y, Kim KS, Bergey EJ, Prasad PN (2002) Chem Mater 14:3715–3721CrossRefGoogle Scholar
  16. 16.
    Dai H, Zhu J, Yang ZJ, Li J, Jiao XA, Hu XY, Wang J (2013) Chem Commun 49:1708–1710CrossRefGoogle Scholar
  17. 17.
    Qi D, Deng CH, Zhang XG, Zhao DY (2008) J Am Chem Soc 130:2829–2840CrossRefGoogle Scholar
  18. 18.
    Lu Y, Yin Y, Mayers BT, Xia Y (2002) Nano Lett 2:183–186CrossRefGoogle Scholar
  19. 19.
    Deng YH, Wang CC, Hu JH, Yang WL, Fu SK (2005) Colloid Surf A 26:87–93CrossRefGoogle Scholar
  20. 20.
    Feng JJ, Li ZH, Li YF, Wang AJ, Zhang PP (2012) Microchim Acta 176:201–208CrossRefGoogle Scholar
  21. 21.
    Wang AJ, Li YF, Li ZH, Feng JJ, Sun YL, Chen JR (2012) Mater Sci Eng C 32:1640–1647CrossRefGoogle Scholar
  22. 22.
    Bindhu LV, Abraham ET (2003) J Appl Polym Sci 88:1456–1464CrossRefGoogle Scholar
  23. 23.
    Yang ZJ, Shen J, Li J, Zhu J, Hu XY (2013) Anal Chim Acta 774:85–91CrossRefGoogle Scholar
  24. 24.
    Qi DW, Lu J, Deng CH, Zhang XM (2009) J Phys Chem C113:15854–15861Google Scholar
  25. 25.
    Kauppien JK, Moffatt DJ, Mantsch HH, Cameron DG (1981) Appl Spectrosc 35:271–276CrossRefGoogle Scholar
  26. 26.
    Song YP, Petty MC, Yarwood J, Feast WJ, Tsibouklis J, Mukherjee S (1992) Langmuir 8:257–261CrossRefGoogle Scholar
  27. 27.
    Bard AJ, Faulkner LR (1980) Electrochemical Methods. Wiley, New YorkGoogle Scholar
  28. 28.
    Laviron E (1979) J Electroanal Chem 101:19–28CrossRefGoogle Scholar
  29. 29.
    Sun W, Li XQ, Qin P, Jiao K (2009) J Phys Chem C 113:11294–11300CrossRefGoogle Scholar
  30. 30.
    Liu CY, Hu JM (2009) Biosens Bioelectron 24:2149–2154CrossRefGoogle Scholar
  31. 31.
    Moghaddam AB, Ganjali MR, Dinarvand R, Ahadi S, Saboury AA (2008) Biophy Chem 134:25–33CrossRefGoogle Scholar
  32. 32.
    Fan CH, Zhuang Y, Li GX, Zhu JQ, Zhu DX (2007) Electroanalysis 12:1156–1158CrossRefGoogle Scholar
  33. 33.
    He PL, Hu NF, Zhou G (2002) Biomacromolecules 3:139–146CrossRefGoogle Scholar
  34. 34.
    Sun H, Hu NF (2005) Analyst 130:76–84CrossRefGoogle Scholar
  35. 35.
    Kamin RA, Wilson GS (1980) Anal Chem 52:1198–1205CrossRefGoogle Scholar
  36. 36.
    Sun W, Li XQ, Jiao K (2009) Electroanalysis 21:959–964CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Xiaofeng Wang
    • 1
  • Zheng You
    • 1
  • Hailiang Sha
    • 2
  • Zhaolan Sun
    • 3
  • Wei Sun
    • 3
    Email author
  1. 1.Department of Precision InstrumentsTsinghua UniversityBeijingPeople’s Republic of China
  2. 2.Department of OrthodonticsBeijing Stomatological HospitalBeijingPeople’s Republic of China
  3. 3.College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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