Journal of Solid State Electrochemistry

, Volume 18, Issue 1, pp 207–213 | Cite as

Electrochemical myoglobin biosensor based on carbon ionic liquid electrode modified with Fe3O4@SiO2 microsphere

  • Xiaofeng Wang
  • Zheng You
  • Hailiang Sha
  • Zhaolan Sun
  • Wei SunEmail author
Original Paper


In this paper, a Fe3O4@SiO2 core-shell structure microsphere was synthesized and used to investigate the direct electron transfer of myoglobin (Mb) with a 1-butylpyridinium hexafluorophosphate based carbon ionic liquid electrode (CILE) as the substrate electrode. The mixture of Mb and Fe3O4@SiO2 microsphere could form an organic–inorganic composite, which was immobilized on the surface of CILE with a chitosan (CS) film. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared on CS/Mb-Fe3O4@SiO2/CILE with the formal peak potential (E 0′) located at −0.31 V (vs. saturated calomel electrode), which was corresponded to the electroactive center of Mb heme Fe(III)/Fe(II) redox couples. Direct electrochemical behaviors of Mb in CS-Fe3O4@SiO2 composite film were carefully investigated with the electrochemical parameters calculated. The CS/Mb-Fe3O4@SiO2/CILE showed good electrocatalytic behaviors to the reduction of trichloroacetic acid in the concentration range from 0.2 to 11.0 mmol L−1 with the detection limit of 0.18 mmol L−1 (3σ). Based on CS/Mb-Fe3O4@SiO2/CILE, a new third-generation reagentless electrochemical biosensor was constructed with higher sensitivity and reproducibility.


1-Butylpyridinium hexafluorophosphate Myoglobin Direct electrochemistry Carbon ionic liquid electrode Fe3O4@SiO2 microsphere 



We are grateful for the financial support of the National Natural Science Foundation of China (nos. 30901700, 50905096).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Xiaofeng Wang
    • 1
  • Zheng You
    • 1
  • Hailiang Sha
    • 2
  • Zhaolan Sun
    • 3
  • Wei Sun
    • 3
    Email author
  1. 1.Department of Precision InstrumentsTsinghua UniversityBeijingPeople’s Republic of China
  2. 2.Department of OrthodonticsBeijing Stomatological HospitalBeijingPeople’s Republic of China
  3. 3.College of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdaoPeople’s Republic of China

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