Enantioselective recognition of penicillamine enantiomers on bovine serum albumin-modified glassy carbon electrode
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As a natural chiral selector, bovine serum albumin (BSA) has been used to recognize penicillamine (Pen) enantiomers through electrochemical methods. The recognition and assay rely on the stereoselectivity of BSA embedded in ultrathin Al2O3 sol–gel film coated on the surface of glassy carbon electrode (BSA/GCE). The enantioselective interaction between Pen enantiomers and BSA was monitored by cyclic voltammetry and electrochemical impedance spectroscopy measurements, from which larger response signals were obtained from d-Pen. The factors influencing the performance of the modified biosensor were also investigated. The association constant (K) was calculated to be 1.93 × 104 L mol−1 for d-Pen and 1.20 × 103 L mol−1 for l-Pen. A good linear response was exhibited with the concentration of Pen enantiomers by BSA/GCE over the range of 1 × 10−8–1 × 10−1 mol L−1 with a detection limit of 3.31 × 10−9 mol L−1.
KeywordsBovine serum albumin Penicillamine enantiomers Stereoselectivity Chiral recognition
This work was supported by the National Natural Science Foundation of China (20972128).
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