Journal of Solid State Electrochemistry

, Volume 16, Issue 12, pp 3747–3752

Direct electrochemistry of glucose oxidase immobilized on TiO2–graphene/nickel oxide nanocomposite film and its application

  • Chun-Xuan Xu
  • Ke-Jing Huang
  • Xue-Min Chen
  • Xiao-Qin Xiong
Original Paper


A novel electrochemical platform based on nickel oxide (NiO) nanoparticles and TiO2–graphene (TiO2–Gr) was developed for the direct electrochemistry of glucose oxidase (GOD). The electrochemical behavior of the sensor was studied using cyclic voltammetry and chronoamperometry. The experimental results demonstrated that the nanocomposite well retained the activity of GOD and the modified electrode GOD/NiO/TiO2–Gr/GCE exhibited excellent electrocatalytic activity toward the redox of GOD as evidenced by the significant enhancement of redox peak currents in comparison with bare GCE. The biosensor responded linearly to glucose in the range of 1.0–12.0 mM, with a sensitivity of 4.129 μA mM−1 and a detection limit of 1.2 × 10−6 M under optimized conditions. The response time of the biosensor was 3 s. In addition, the developed biosensor possessed good reproducibility and stability, and there was negligible interference from other electroactive components.


TiO2–graphene Nickel oxide nanoparticles Glucose Glucose oxidase Biosensor 


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Chun-Xuan Xu
    • 1
  • Ke-Jing Huang
    • 1
  • Xue-Min Chen
    • 1
  • Xiao-Qin Xiong
    • 1
  1. 1.College of Chemistry and Chemical EngineeringXinyang Normal UniversityXinyangChina

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