α-MoO3 nanowire-based amperometric biosensor for l-lactate detection
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Large-scale orthorhombic single-crystalline molybdenum trioxide nanowires were synthesized using a facile one-pot hydrothermal method. Lactate oxidase enzyme was immobilized on the nanowires to produce a highly sensitive electrochemical biosensor for l-lactate detection. At an applied potential of 0.5 V, the sensor exhibited a high sensitivity of 0.87 μA/mM with a fast response to l-lactate (90% of response times within 10 s). A linear response was obtained over a concentration range from 0.5 to 8 mM with a detection limit of 0.15 mM (S/N = 3). The developed biosensor showed excellent reproducibility and operational stability, as well as the ability to be stored long term.
KeywordsBiosensor l-lactate MoO3 nanowires Direct electron transfer
This work was supported by the Korean Ministry of Education, Science and Technology under grants NRF-2011-0031392 (Priority Research Centers Program), R31-2008-000-10029-0 (World Class University Program), and S-2011-0292-000 (Basic Science Research Program) and by the Global Frontier Research Center for Advanced Soft Electronics.
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