Journal of Solid State Electrochemistry

, Volume 16, Issue 7, pp 2315–2328 | Cite as

Protein film voltammetry: electrochemical enzymatic spectroscopy. A review on recent progress

  • Rubin Gulaboski
  • Valentin Mirčeski
  • Ivan Bogeski
  • Markus Hoth


This review is focused on the basic principles, the main applications, and the theoretical models developed for various redox mechanisms in protein film voltammetry, with a special emphasis to square-wave voltammetry as a working technique. Special attention is paid to the thermodynamic and kinetic parameters of relevant enzymes studied in the last decade at various modified electrodes, and their use as a platform for the detection of reactive oxygen species is also discussed. A set of recurrent formulas for simulations of different redox mechanisms of lipophilic enzymes is supplied together with representative simulated voltammograms that illustrate the most relevant voltammetric features of proteins studied under conditions of square-wave voltammetry.


Redox enzymes Electrochemistry Modified electrodes Reactive oxygen species Mathematical modelling 



R.G. thanks the Alexander von Humboldt Foundation for providing a Return postdoctoral fellowship. This work is also supported by the Alexander von Humboldt Foundation via the joint German–Macedonian project from the Research Group Linkage Programme 3.4-Fokoop-DEU/1128670 (to V.M., R.G. I.B., and M.H.). M.H. also acknowledges the support by the Deutsche Forschungsgemeinschaft (SFB 530, SFB 894, GK 845, GK 1326).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Rubin Gulaboski
    • 1
  • Valentin Mirčeski
    • 2
  • Ivan Bogeski
    • 3
  • Markus Hoth
    • 3
  1. 1.Faculty of Agriculture, Department of Chemistry, Goce Delčev UniversityŠtipMacedonia
  2. 2.Faculty of Natural Sciences and Mathematics, Institute of Chemistry“Ss Kiril i Metodij” UniversitySkopjeMacedonia
  3. 3.Faculty of Medicine, Department of BiophysicsUniversity of SaarlandHomburgGermany

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