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Molecular modeling in the age of clinical genomics, the enterprise of the next generation

  • Jeremy W. ProkopEmail author
  • Jozef Lazar
  • Gabrielle Crapitto
  • D. Casey Smith
  • Elizabeth A. Worthey
  • Howard J. Jacob
Original Paper

Abstract

Protein modeling and molecular dynamics hold a unique toolset to aide in the characterization of clinical variants that may result in disease. Not only do these techniques offer the ability to study under characterized proteins, but they do this with the speed that is needed for time-sensitive clinical cases. In this paper we retrospectively study a clinical variant in the XIAP protein, C203Y, while addressing additional variants seen in patients with similar gastrointestinal phenotypes as the C203Y mutation. In agreement with the clinical tests performed on the C203Y patient, protein modeling and molecular dynamics suggest that direct interactions with RIPK2 and Caspase3 are altered by the C203Y mutation and subsequent loss of Zn coordination in the second BIR domain of XIAP. Interestingly, the variant does not appear to alter interactions with SMAC, resulting in further damage to the caspase and NOD2 pathways. To expand the computational strategy designed when studying XIAP, we have applied the molecular modeling tools to a list of 140 variants seen in CFTR associated with cystic fibrosis, and a list of undiagnosed variants in 17 different genes. This paper shows the exciting applications of molecular modeling in the classification and characterization of genetic variants identified in next generation sequencing.

Graphical abstract

XIAP in Caspase 3 and NOD2 signaling pathways

Keywords

XIAP Caspase3 SMAC RIPK2 Clinical variants Protein modeling Undiagnosed and rare diseases 

Supplementary material

894_2017_3258_MOESM1_ESM.zip (14.3 mb)
ESM 1 (ZIP 14635 kb)
894_2017_3258_MOESM2_ESM.xlsx (42 kb)
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894_2017_3258_MOESM3_ESM.xlsx (23 kb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Jeremy W. Prokop
    • 1
    • 2
    Email author
  • Jozef Lazar
    • 1
    • 2
  • Gabrielle Crapitto
    • 2
  • D. Casey Smith
    • 1
  • Elizabeth A. Worthey
    • 1
    • 2
  • Howard J. Jacob
    • 1
    • 2
  1. 1.HudsonAlpha Institute for BiotechnologyHuntsvilleUSA
  2. 2.Human and Molecular Genetics CenterMedical College of WisconsinMilwaukeeUSA

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