Journal of Molecular Modeling

, Volume 19, Issue 11, pp 4969–4989

Destabilization of the MutSα’s protein-protein interface due to binding to the DNA adduct induced by anticancer agent carboplatin via molecular dynamics simulations

Original Paper

DOI: 10.1007/s00894-013-1998-2

Cite this article as:
Negureanu, L. & Salsbury, F.R. J Mol Model (2013) 19: 4969. doi:10.1007/s00894-013-1998-2

Abstract

DNA mismatch repair (MMR) proteins maintain genetic integrity in all organisms by recognizing and repairing DNA errors. Such alteration of hereditary information can lead to various diseases, including cancer. Besides their role in DNA repair, MMR proteins detect and initiate cellular responses to certain type of DNA damage. Its response to the damaged DNA has made the human MMR pathway a useful target for anticancer agents such as carboplatin. This study indicates that strong, specific interactions at the interface of MutSα in response to the mismatched DNA recognition are replaced by weak, non-specific interactions in response to the damaged DNA recognition. Data suggest a severe impairment of the dimerization of MutSα in response to the damaged DNA recognition. While the core of MutSα is preserved in response to the damaged DNA recognition, the loss of contact surface and the rearrangement of contacts at the protein interface suggest a different packing in response to the damaged DNA recognition. Coupled in response to the mismatched DNA recognition, interaction energies, hydrogen bonds, salt bridges, and solvent accessible surface areas at the interface of MutSα and within the subunits are uncoupled or asynchronously coupled in response to the damaged DNA recognition. These pieces of evidence suggest that the loss of a synchronous mode of response in the MutSα’s surveillance for DNA errors would possibly be one of the mechanism(s) of signaling the MMR-dependent programed cell death much wanted in anticancer therapies. The analysis was drawn from dynamics simulations.

Keywords

MD simulations Mismatch repair proteins MMR Platinum-DNA adducts carboplatin-induced damage DNA recognition Protein-protein interface destabilization 

Supplementary material

894_2013_1998_MOESM1_ESM.pdf (763 kb)
ESM 1(PDF 763 kb)
894_2013_1998_MOESM2_ESM.doc (36 kb)
ESM 2(DOC 35 kb)
894_2013_1998_MOESM3_ESM.pdf (266 kb)
ESM 3(PDF 266 kb)
894_2013_1998_MOESM4_ESM.pdf (1.8 mb)
ESM 4(PDF 1812 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of PhysicsWake Forest UniversityWinston SalemUSA

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