Molecular association of FtsZ with the intrabacterial nanotransportation system for urease in Helicobacter pylori
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Helicobacter pylori possesses intrabacterial nanotransportation system (ibNoTS) for transporting CagA, VacA, and urease within the bacterial cytoplasm, which is controlled by the extrabacterial environment. The route of ibNoTS for CagA is reported to be associated with the MreB filament, whereas the route of ibNoTS for urease is not yet known. In this study, we demonstrated by immunoelectron microscopy that urease along the route of ibNoTS localizes closely with the FtsZ filament in the bacterium. Supporting this, we found by enzyme immunoassay and co-immunoprecipitation analysis that urease interacted with FtsZ. These findings indicate that urease along the route of ibNoTS is closely associated with the FtsZ filament. Since these phenomena were not observed in ibNoTS for CagA, the route of ibNoTS for CagA is different from that of ibNoTS for urease. We propose that the route of ibNoTS for urease is associated with the FtsZ filament in H. pylori.
KeywordsHelicobacter pylori FtsZ Urease Intrabacterial nanotransportation system Immunoelectron microscopy
We thank Mr. Yoshihiko Fujioka and Ms. Yukiko Takada of the Department of Microbiology and Infection Control, Osaka Medical College, for their technical help. We also thank Ms. Hiromi Norimitsu for her help in the preparation of the manuscript.
- 5.Gerits N, Mikalsen T, Kostenko S, Shiryaev A, Johannessen M, Moens U (2007) Modulation of F-actin rearrangement by the cyclic AMP/cAMP-dependent protein Kinase (PKA) pathway is mediated by MAPK-activated protein kinase 5 and requires PKA-induced nuclear export of MK5. J Biol Chem 282:37232–37243PubMedGoogle Scholar
- 15.Nakano T, Aoki H, Wu H, Fujioka Y, Nakazawa E, Sano K (2012) Fine visualization of filamentous structures in the bacterial cytoplasm. J Microbiol Meth 90:60–64Google Scholar
- 28.Hong W, Morimatsu S, Goto T, Sachs G, Scott DR, Weeks DL, Kohno T, Morita C, Nakano T, Fujioka Y, Sano K (2000) Contrast-enhanced immunoelectron microscopy for Helicobacter pylori. J Microbiol Meth 42:121–127Google Scholar
- 29.Wu H (2016) Optimum number of bacterial cells for examination of localization of intrabacterial nanotransportation system by semiquantitative immunoelectron microscopy. Bull OMC 62:19–23Google Scholar