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Extremophiles

, Volume 6, Issue 3, pp 201–207 | Cite as

Acidophiles of saline water at thermal vents of Vulcano, Italy

  • Susan Simmons
  • Paul Norris
Original paper

Abstract.

DNA was extracted from samples taken from close to acidic hydrothermal vents on shore of the Aeolian Island of Vulcano (Italy). RNA gene sequences were amplified by PCR, cloned, and sequenced. A sequence with an origin in samples at 35° and 45°C corresponded to that of a novel Acidithiobacillus species that was isolated from water close to the vents. Novel, iron-oxidizing mesophilic acidophiles were isolated through enrichment cultures with ferrous iron but were not represented in the clone banks of environmental rDNA. These acidophiles were related to Thiobacillus prosperus, which was isolated previously from Vulcano. The archaeal sequences that comprised a clone bank representing a high-temperature sample (75°C) corresponded to those of Acidianus brierleyi and of thermophiles previously isolated from Vulcano, Thermoplasma volcanium and Acidianus infernus.

Acidophiles Vulcano Hydrothermal vents 16S rDNA 

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Susan Simmons
    • 1
  • Paul Norris
    • 1
  1. 1.Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK

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