Advertisement

Extremophiles

, Volume 6, Issue 1, pp 39–44 | Cite as

Sulfolobus tokodaii sp. nov. (f. Sulfolobus sp. strain 7), a new member of the genus Sulfolobus isolated from Beppu Hot Springs, Japan

  • Toshiharu Suzuki
  • Toshio Iwasaki
  • Taketoshi Uzawa
  • Kurt Hara
  • Naoki Nemoto
  • Takahide Kon
  • Toshiaki Ueki
  • Akihiko Yamagishi
  • Tairo Oshima
Original paper

Abstract.

The taxonomic position of a thermoacidophilic crenarchaeote Sulfolobus sp. strain 7, previously isolated from the Beppu Hot Springs in the geothermal area of Kyushu Island, Japan, was investigated by cloning and sequencing, by phylogenetic analysis of the 16S rRNA gene sequence, by DNA–DNA homology with similar species, and by biochemical characterization of the isolate. This isolate is an obligate aerobe and grows optimally at 80°C and pH 2.5–3 under aerobic and chemoheterotrophic growth conditions by aerobic respiration rather than simple fermentation. In conjunction with the phenotypic properties, the present phylogenetic analysis based on the 16S rRNA gene sequence and DNA–DNA hybridization experiments indicate that this isolate is related to the described Sulfolobus taxon and should be considered a novel species of the genus. We propose that this isolate is a novel species of the genus Sulfolobus that we name Sulfolobus tokodaii sp. nov. The type strain is strain 7 (JCM 10545).

Sulfolobus tokodaii sp. nov. Archaea Thermoacidophile Crenarchaeote 16S rDNA Phylogeny 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Toshiharu Suzuki
    • 1
  • Toshio Iwasaki
    • 3
  • Taketoshi Uzawa
    • 4
  • Kurt Hara
    • 2
  • Naoki Nemoto
    • 2
  • Takahide Kon
    • 1
  • Toshiaki Ueki
    • 1
  • Akihiko Yamagishi
    • 2
  • Tairo Oshima
    • 2
  1. 1.Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
  2. 2.Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Horinouchi, Tokyo 192-0392, Japan
  3. 3.Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan
  4. 4.Division of Natural Science, Department of Arts and Sciences, Osaka Kyoiku University, Osaka, Japan

Personalised recommendations