Extremophiles

, Volume 5, Issue 5, pp 343–349 | Cite as

Assignment of Pseudomonas sp. strain E-3 to Pseudomonas psychrophila sp. nov., a new facultatively psychrophilic bacterium

  • Isao Yumoto
  • Teruo Kusano
  • Tomohiro Shingyo
  • Yoshinobu Nodasaka
  • Hidetoshi Matsuyama
  • Hidetoshi Okuyama
Original paper

Abstract.

A facultatively psychrophilic bacterium, previously described as Pseudomonas sp. strain E-3, has been reassigned by phenotypic characterization, chemotaxonomic analysis, DNA–DNA hybridization, and 16S rRNA gene phylogenetic analysis. The organism was a gram-negative, aerobic, straight rod with polar flagella. It was catalase positive and oxidase positive, able to grow at –1°C but not at 40°C, and produced acid from D-glucose under aerobic conditions. The major isoprenoid quinone was ubiquinone-9, and the DNA G+C content was 57.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the bacterium is a member of the genus Pseudomonas and was closest to Pseudomonas fragi. Determination of the DNA–DNA relatedness between strain E-3 and P. fragi revealed too low a level of homology (47.9%–51.3%) to identify them as the same species. On the basis of phenotypic characteristics, phylogenetic analysis, and DNA–DNA relatedness data, it is concluded that strain E-3 represents an individual species. Accordingly, the name Pseudomonas psychrophila is proposed. The type strain is E-3T (=JCM 10889).

Pseudomonas psychrophila Facultatively psychrophilic 16S rRNA DNA–DNA hybridization 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Isao Yumoto
    • 1
  • Teruo Kusano
    • 2
  • Tomohiro Shingyo
    • 1
  • Yoshinobu Nodasaka
    • 4
  • Hidetoshi Matsuyama
    • 3
  • Hidetoshi Okuyama
    • 1
  1. 1.Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
  2. 2.Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan
  3. 3.Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Sapporo, Japan
  4. 4.Laboratory of Electron Microscopy, School of Dentistry, Hokkaido University, Sapporo, Japan

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