Extremophiles

, Volume 4, Issue 6, pp 373–376

Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

  • Gudrun Amann
  • K. O. Stetter
  • Enric Llobet-Brossa
  • Rudolf Amann
  • Josefa Antón
ORIGINAL PAPER

Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

Key words 16S rDNA heterogeneity Archaea Haloarcula marismortui Fluorescence in situ hybridization 

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Copyright information

© Springer-Verlag Tokyo 2000

Authors and Affiliations

  • Gudrun Amann
    • 1
  • K. O. Stetter
    • 1
  • Enric Llobet-Brossa
    • 2
  • Rudolf Amann
    • 2
  • Josefa Antón
    • 2
  1. 1.Lehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany Tel. +49-941-9433161; Fax +49-941-9432403 e-mail: karl.stetter@biologie.uni-regensburg.deDE
  2. 2.Max-Planck Institute for Marine Microbiology, Bremen, GermanyDE
  3. 3.División de Microbiología, Universidad de Alicante, Alicante, SpainES

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