, Volume 4, Issue 2, pp 83–90 | Cite as

Toward a molecular understanding of cold activity of enzymes from psychrophiles

  • N. J. Russell


Despite the fact that a much greater proportion of the earth environment is cold rather than hot, much less is known about psychrophilic, cold-adapted microorganisms compared with thermophiles living at high temperatures. In particular, investigation of the molecular basis of cold-active enzymes from psychrophiles has only recently received concerted research attention, in measure as a result of the EC-funded project COLDZYME. This research effort has been stimulated by the realization that such cold-active enzymes offer novel opportunities for biotechnological exploitation. Only very recently has the first cold-active enzyme, α-amylase, been crystallized, and this success was followed rapidly by others. This effort has facilitated a direct approach to solving the three-dimensional structure of cold-active enzymes to complement the gene homology modeling that had been performed previously. Recently studies have highlighted how different adaptations are used by different enzymes to achieve conformational flexibility at low temperatures, and how such adaptations are not necessarily the opposite of those that confer thermostability to proteins in thermophilic counterparts. This review also highlights initial successes in engineering genetically improved thermal stability in cold-active enzymes to give improved catalysts for low-temperature biotechnology.

Key words Psychrophilic bacteria Cold adaptation Cold-active enzymes Protein structure Molecular conformation Biotechnology 


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Copyright information

© Springer-Verlag Tokyo 2000

Authors and Affiliations

  • N. J. Russell
    • 1
  1. 1.Microbiology Laboratories, Department of Biological Sciences, Wye College University of London, Wye, Ashford, Kent TN25 5AH, UK Tel. +44-1233-812401 (ext 411); Fax +44-1233-813140, e-mail: n.russell@wye.ac.ukGB

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