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Extremophiles

, Volume 3, Issue 4, pp 293–296 | Cite as

Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans

  • Hideto Takami
  • Yuichi Nogi
  • Koki Horikoshi
ORIGINAL PAPER

Abstract

Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA–DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA–DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA–DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans.

Key words Alkaliphilic Bacillus halodurans AH-101 DNA–DNA hybridization Phylogenetic tree Identification 

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Copyright information

© Springer-Verlag Tokyo 1999

Authors and Affiliations

  • Hideto Takami
    • 1
  • Yuichi Nogi
    • 1
  • Koki Horikoshi
    • 1
  1. 1.Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jpJP

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