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Extremophiles

, Volume 3, Issue 2, pp 139–145 | Cite as

Novel archaeal phylotypes from an East African alkaline saltern

  • S. Grant
  • William D. Grant
  • Brian E. Jones
  • Chiaki Kato
  • Lina Li

Abstract

DNA has been extracted on site from the brines of the final crystallizing pond of an alkaline saltern at Lake Magadi, Kenya. Amplification of 16S rRNA genes followed by cloning, sequencing, and phylogenetic analysis has revealed the presence of two distinct new archaeal lineages. The majority of cloned sequences showed greater than 95% identity to each other, but only 88%–90% similarity to any cultivated haloalkaliphilic Archaea, and form a distinct cluster within the known Haloarchaea. Two cloned genes showed close similarity to each other but only 76% similarity to any known archaeal sequence, and therefore represent a distinct phylotype only distantly related to the euryarchaeotal branch of the Archaea.

Key words Halophile Haloalkaliphile Phylogeny Phylotype 16S rDNA Alkaline saltern Archaea 

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Copyright information

© Springer-Verlag Tokyo 1999

Authors and Affiliations

  • S. Grant
    • 1
  • William D. Grant
    • 1
  • Brian E. Jones
    • 2
  • Chiaki Kato
    • 3
  • Lina Li
    • 3
  1. 1.Department of Microbiology and Immunology, University of Leicester, PO Box 138, Leicester LE1 9HN, UK Tel. +44-116 252 2940; Fax +44-116 252 5030 e-mail: sg23@le.ac.ukGB
  2. 2.Genencor International BV, P.O. Box 642, 2600 AP Delft, The NetherlandsNL
  3. 3.The DEEPSTAR Group, Japan Marine Science and Technology Centre, 2-15 Natsushima-cho, Yokosuka 237-0061, JapanJP

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