Extremophiles

, Volume 2, Issue 4, pp 401–407

Characterization of α-maltotetraohydrolase produced by Pseudomonas sp. MS300 isolated from the deepest site of the Mariana Trench

  • H. Kobayashi
  • Yoshihiro Takaki
  • Kuniko Kobata
  • Hideto Takami
  • Akira Inoue
  • 69 Downloads

Abstract

We have isolated a Pseudomonas-like amylase producer, the strain MS300, which displayed a large halo on starch medium, from the deepest site of the Mariana Trench. The strain MS300 produced two major and two minor α-maltotetraohydrolases (G4-amylase). The two major G4-amylases share the same molecular weight of 55 000 but had different pI values, 5.0 and 4.7, respectively. The optimum temperature for activity of both major G4-amylases is 40°C, and the optimum pH is 6.8 for one and 8.9 for the other. MS300 produced more amylase under high hydrostatic pressure than under atmospheric pressure. Strain MS300 may be active in the deep sea at a depth of 10 897 m.

Key wordsα-Maltotetraohydrolase Mariana Trench High hydrostatic pressure Deep sea Amylase 

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Copyright information

© Springer-Verlag Tokyo 1998

Authors and Affiliations

  • H. Kobayashi
    • 1
  • Yoshihiro Takaki
    • 1
  • Kuniko Kobata
    • 1
  • Hideto Takami
    • 1
  • Akira Inoue
    • 1
  1. 1.Japan Marine Science and Technology Center, Deep-Sea Microorganism Research Group, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-5556; Fax +81-468-66-6364 e-mail: hidekik@jamstec.go.jpJP

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