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Extremophiles

, Volume 2, Issue 2, pp 67–74 | Cite as

Bioenergetics and cytoplasmic membrane stability of the extremely acidophilic, thermophilic archaeon Picrophilus oshimae

  • Jack L. C. M. van de Vossenberg
  • Arnold J. M. Driessen
  • Wolfram Zillig
  • W. N. Konings
ORIGINAL PAPER

Abstract

Picrophilus oshimae is an extremely acidophilic, thermophilic archaeon that grows optimally at 60°C and at pH 0.7. It is an obligatory acidophile that does not grow at pH values above 4.0. The proton motive force in respiring cells is composed of a large transmembrane pH gradient, inside less acid, and a reversed transmembrane electrical potential, inside positive. Cells maintain an intracellular pH at around 4.6 at extracellular pH values ranging from 0.8 to 4.0. Above pH 4.0 cells lyse rapidly and lose their viability. Liposomes prepared from lipids derived from P. oshimae have an extremely low proton permeability at acidic pH. However, at neutral pH, the lipids are unable to assemble into regular liposomal structures. These observations suggest that the loss of viability and cell integrity above pH 4.0 is due to an impairment of the barrier function of the cytoplasmic membrane.

Key wordsPicrophilus oshimae Cytoplasmic membrane Permeability Solute transport 

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Copyright information

© Springer-Verlag Tokyo 1998

Authors and Affiliations

  • Jack L. C. M. van de Vossenberg
    • 1
  • Arnold J. M. Driessen
    • 1
  • Wolfram Zillig
    • 2
  • W. N. Konings
    • 1
  1. 1.Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands Tel. +31-50-3632150; Fax +31-50-3632154 e-mail: W.N.Konings@biol.rug.nlNL
  2. 2.Max Planck Institut für Biochemie, Am Klopferspitz 18A, 82152 Martinsried, Germany DE

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