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Pelomicrobium methylotrophicum gen. nov., sp. nov. a moderately thermophilic, facultatively anaerobic, lithoautotrophic and methylotrophic bacterium isolated from a terrestrial mud volcano

  • G. B. SlobodkinaEmail author
  • A. Y. Merkel
  • A. A. Novikov
  • E. A. Bonch-Osmolovskaya
  • A. I. Slobodkin
Original Paper
  • 2 Downloads

Abstract

A novel moderately thermophilic, bacterium, strain SM250T, was isolated from a terrestrial mud volcano, Taman peninsula, Krasnodar region, Russia. Cells of strain SM250T were Gram-negative non-spore forming motile straight rods. Growth was observed at temperatures 30–63 °C (optimum at 50 °C), pH 6.5–10.0 (optimum at pH 8.5) and NaCl concentrations 0–4.5% (w/v) (optimum at 1.0–1.5% (w/v)). The novel isolate grows by aerobic respiration or anaerobic respiration with nitrate as the terminal electron acceptor. Strain SM250T grows by the utilization of methanol, formate and a number of other organic compounds or lithoautotrophically with hydrogen, elemental sulfur or thiosulfate as electron donors. The total size of the genome of the novel isolate was 3,327,116 bp and a genomic DNA G + C content was 64.8 mol%. Analysis of the 16S rRNA gene sequences revealed that strain SM250T belongs to the class Hydrogenophilia within the phylum Proteobacteria, with less than 91% of 16S rRNA gene sequence similarity to any species with validly published name. We propose to assign strain SM250T to a new species of a novel genus Pelomicrobium methylotrophicum gen. nov., sp. nov. The type strain is SM250T (= KCTC 62861T = VKM B-3274T).

Keywords

Hydrogen oxidation Sulfur oxidation Nitrate reduction CO2 fixation Proteobacteria Hydrogenophilales 

Notes

Acknowledgements

The work of G.B.S., A.I.S. and E.A.B.-O. was supported by the Russian Science Foundation, project no. 17-74-30025. The work of A.Y.M (genome sequencing and assembly) was supported by the Ministry of Science and Higher Education of the Russian Federation. The work of A.A.N (chemotaxonomic studies) was supported by the President of Russia (grant MК-3801.2018.4; agreement 075-15-2019-1025.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • G. B. Slobodkina
    • 1
    Email author
  • A. Y. Merkel
    • 1
  • A. A. Novikov
    • 2
  • E. A. Bonch-Osmolovskaya
    • 1
  • A. I. Slobodkin
    • 1
  1. 1.Winogradsky Institute of MicrobiologyResearch Center of Biotechnology of the Russian Academy of SciencesMoscowRussia
  2. 2.Gubkin UniversityMoscowRussia

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