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Bioremediation potential of a halophilic Halobacillus sp. strain, EG1HP4QL: exopolysaccharide production, crude oil degradation, and heavy metal tolerance

  • Ibrahim M. Ibrahim
  • Svetlana A. Konnova
  • Elena N. Sigida
  • Elena V. Lyubun
  • Anna Yu. Muratova
  • Yulia P. FedonenkoEmail author
  • Кhaled Elbanna
Original Paper
  • 6 Downloads

Abstract

A halophilic bacterial strain, EG1HP4QL, was isolated from a salt sample from Lake Qarun, Fayoum Province, Egypt. Morphological, physiological, biochemical, and phylogenetic analyses indicated that the strain belonged to the genus Halobacillus. Strain EG1HP4QL produced an extracellular polysaccharide (EPS), with production peaking (5.9 g L−1) during growth on medium S-G containing 2% (w/v) sucrose at 35 °C (pH 8.0). The EPS had significant emulsifying activity (E24 %) against kerosene (65.7 ± 0.8%), o-xylene (64.0 ± 1%), and sunflower oil (44.7 ± 0.5%). The composition of the EPS included two polymers—a negatively charged and a neutral one (~ 3:1)—in which mannose and glucose were the main neutral monosaccharide constituents. Strain EG1HP4QL was able to utilize crude oil (35.3%) as the sole carbon source within 12 days. The minimum inhibitory concentrations of heavy metals [Zn(II), Cd(II), Pb(II), Ni(II), and Cu(II)] for strain EG1HP4QL were 1.0, 2.0, 2.0, 2.5, and 5 mM, respectively.

Keywords

Halobacillus sp. Extracellular polysaccharide Crude oil degradation Heavy metal tolerance 

Notes

Acknowledgements

We thank the staff of the Russian Collection of Agricultural Microorganisms of the All-Russia Research Institute for Agricultural Microbiology (St. Petersburg, Russia) for the sequence of the gene 16S rDNA and Dr. Andrey M. Burov from the Simbioz Center for the Collective Use of Research Equipment in the Field of Physical–Chemical Biology and Nanobiotechnology (IBPPM RAS) for technical support in transmission electron microscopy.

Compliance with ethical standards

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Ibrahim M. Ibrahim
    • 1
    • 2
  • Svetlana A. Konnova
    • 1
    • 3
  • Elena N. Sigida
    • 3
  • Elena V. Lyubun
    • 3
  • Anna Yu. Muratova
    • 3
  • Yulia P. Fedonenko
    • 1
    • 3
    Email author
  • Кhaled Elbanna
    • 2
    • 4
  1. 1.Chernyshevsky Saratov State UniversitySaratovRussia
  2. 2.Department of Agricultural Microbiology, Faculty of AgricultureFayoum UniversityFayoumEgypt
  3. 3.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia
  4. 4.Department of Biology, Faculty of Applied ScienceUmm Al-Qura UniversityMakkahKingdom of Saudi Arabia

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