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Extremophiles

, Volume 24, Issue 1, pp 135–145 | Cite as

Cell membrane fatty acid and pigment composition of the psychrotolerant cyanobacterium Nodularia spumigena CHS1 isolated from Hopar glacier, Pakistan

  • Noor Hassan
  • Alexandre M. Anesio
  • Muhammad Rafiq
  • Jens Holtvoeth
  • Ian Bull
  • Christopher J. Williamson
  • Fariha HasanEmail author
Original Paper

Abstract

In the present study, cyanobacterium isolate CHS1 isolated from Hopar glacier, Pakistan, was analyzed for the first time for cell membrane fatty acids and production of pigments. Sequencing of the 16-23S intergenetic region confirmed identification of the isolate CHS1 as Nodularia spumigena. All chlorophyll and carotenoid pigments were quantified using high-performance liquid chromatography and experiments to test tolerance against a range of physico-chemical conditions were conducted. Likewise, the fatty acid profile of the cell membrane CHS1 was analyzed using gas chromatography and mass spectroscopy. The cyanobacterium isolate CHS1 demonstrated tolerance to 8 g/L% NaCl, 35°C and pH 5–9. The characteristic polyunsaturated fatty acid (PUFA) of isolate CHS1, C18:4, was observed in fatty acid methyl esters (FAMEs) extracted from the cell membrane. CHS1 was capable of producing saturated fatty acids (SFA) (e.g., C16:0), monounsaturated fatty acids (MUFA) (e.g., C18:1) and polyunsaturated fatty acids (e.g., C20:5) in the cell membrane. In this study, we hypothesize that one mechanism of cold adaptation displayed by isolate CHS1 is the accumulation of high amounts of PUFA in the cell membrane.

Keywords

Nodularia spumigena CHS1 Cyanobacteria Cell membrane FAMEs Hopar glacier 

Notes

Acknowledgements

The research work was financially supported by Commonwealth Scholarship Commission, United Kingdom. In addition, NERC Life Sciences Mass Spectrometry Facility, School of Chemistry, University of Bristol, also supported this study.

Supplementary material

792_2019_1141_MOESM1_ESM.docx (21 kb)
Supplementary file1 (DOCX 20 kb)
792_2019_1141_MOESM2_ESM.docx (15 kb)
Supplementary file2 (DOCX 15 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Noor Hassan
    • 1
    • 2
  • Alexandre M. Anesio
    • 2
    • 3
  • Muhammad Rafiq
    • 1
    • 4
  • Jens Holtvoeth
    • 5
  • Ian Bull
    • 5
  • Christopher J. Williamson
    • 2
  • Fariha Hasan
    • 1
    Email author
  1. 1.Applied, Environmental and Geomicrobiology Laboratory, Department of MicrobiologyQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Bristol Glaciology Centre, School of Geographical Sciences, Faculty of ScienceUniversity of BristolBristolUK
  3. 3.Department of Environmental ScienceAarhus UniversityRoskildeDenmark
  4. 4.Department of MicrobiologyBalochistan University of Information Technology, Engineering and Management SciencesQuettaPakistan
  5. 5.Organic Geochemistry Unit, School of Chemistry, Faculty of ScienceUniversity of BristolBristolUK

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