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Extremophiles

, Volume 23, Issue 5, pp 521–528 | Cite as

Description of Halegenticoccus soli gen. nov., sp. nov., a halophilic archaeon isolated from a soil sample of Ebi lake

  • Bing-Bing Liu
  • Manik Prabhu Narsing Rao
  • Xiao-Qing Yin
  • Xin Li
  • Nimaichand Salam
  • Yao Zhang
  • Dalal Hussien M. Alkhalifah
  • Wael N. Hozzein
  • Wen-Jun LiEmail author
Original Paper
  • 96 Downloads

Abstract

Two extreme halophilic archaeal strains, SYSUA9-0T and SYSUA9-1, were isolated from Ebi lake of Xinjiang, China. The colonies were Gram-negative, coccoid, and non-motile. Strains were aerobic and grew at 25–50 °C (optimum at 37 °C), in the presence of 10–35% (w/v) NaCl (optimum at 20–22%), and pH 6.0–8.0 (optimum at 7.0). The 16S rRNA gene sequence result revealed that the two strains were closely related to Haloprofundus marisrubri SB9T (92.7% similarity). The DNA–DNA hybridization value (97% ± 1%) suggested that SYSUA9-0T and SYSUA9-1 were similar; however, their sequence similarities with other archaeal members suggested that they were novel candidates. The genomic G + C content of SYSUA9-0T was 66.9%. The average nucleotide identity value between SYSU A9-0T and Haloprofundus marisrubri SB9T was 69.1%, which was far below the cutoff value (95–96%) proposed to define the species boundary. The polar lipids were phosphatidylglycerol (PG), phosphatidylglycerolphosphate methylester (PGP-Me), sulfated mannosyl glucosyl diether, mannosyl glucosyldiether, and four unidentified glycolipids. Phenotypic, chemotaxonomic and comparative genome analysis suggested that SYSU A9-0T and SYSU A9-1 represent a novel species of a new genus within the family Haloferacaceae, for which the name Halegenticoccus soli gen. nov., sp. nov., is proposed. The type strain is SYAUA9-0T (= KCTC4241T = CGMCC 1.15765T).

Keywords

Haloferacaceae Halegenticoccus soli Halophile Archaea Ebi lake 

Notes

Acknowledgements

The authors acknowledge Prof. Dr. Yu-Guang Zhou (CGMCC, China) for providing the reference type strains. This research was supported by National Science and Technology Foundation Project (No. 2017FY100300), National Natural Science Foundation of China (Nos. 91751206, 31800001), China Postdoctoral Science Foundation funded project (2015M580748), China Postdoctoral Special Foundation funded project (2016T90811), Natural Science Foundation of Guangdong Province (2017A030310206) and China Biodiversity Observation Networks (Sino BON). This work was also funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1438-0004).

The 16S rRNA gene sequences of strains SYSU A9-0T and SYSU A9-1 have been deposited in GenBank/EMBL/DDBJ under the accession numbers KX752787and KX752786. The rpoB’ gene sequences of SYSU A9-0T and SYSU A9-1 has been deposited in GenBank/EMBL/DDBJ under the accession numbers KX752788 and KX752789, respectively. The whole genome shotgun project of strain SYSU A9-0T has been deposited at DDBJ/EMBL/GenBank under the accession PEND00000000.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

792_2019_1104_MOESM1_ESM.docx (326 kb)
Supplementary material 1 (DOCX 325 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Bing-Bing Liu
    • 1
  • Manik Prabhu Narsing Rao
    • 2
  • Xiao-Qing Yin
    • 2
  • Xin Li
    • 2
  • Nimaichand Salam
    • 2
  • Yao Zhang
    • 2
  • Dalal Hussien M. Alkhalifah
    • 4
  • Wael N. Hozzein
    • 5
    • 6
  • Wen-Jun Li
    • 2
    • 3
    Email author
  1. 1.Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, College of Biological and Chemical EngineeringNanyang Institute of TechnologyNanyangPeople’s Republic of China
  2. 2.State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  3. 3.Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesÜrűmqiChina
  4. 4.Biology Department, Faculty of SciencePrincess Nourah Bint Abdulrahman UniversityRiyadhKingdom of Saudi Arabia
  5. 5.Bioproducts Research Chair, Zoology Department, College of ScienceKing Saud UniversityRiyadhKingdom of Saudi Arabia
  6. 6.Botany and Microbiology Department, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt

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