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Production, characterization and antimicrobial activities of bio-pigments by Aquisalibacillus elongatus MB592, Salinicoccus sesuvii MB597, and Halomonas aquamarina MB598 isolated from Khewra Salt Range, Pakistan

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Abstract

Hypersaline ecosystems offer unique habitats to microbial populations capable of withstanding extreme stress conditions and producing novel metabolites of commercial importance. Herein, we have characterized for the first time the production of bioactive pigments from newly isolated halophilic bacterial species. Halophilic bacteria were isolated from Khewra Salt Range of Pakistan. Three distinctly colored isolates were selected for pigment production. Selected colonies were identified as Aquisalibacillus elongatus MB592, Salinicoccus sesuvii MB597, and Halomonas aquamarina MB598 based on morphological, biochemical, and physiological evidences as well as 16S rRNA analysis. The optimum pigment production observed at mesophilic condition, nearly neutral pH, and moderate salinity was validated using response surface methodology. Different analytical techniques (UV spectroscopy, infrared spectroscopy, and HPLC) characterized these purified pigments as derivatives of bacterioruberin carotenoids. Antioxidant activity of pigments revealed up to 85% free-radical scavenging activity at the concentration of 30 µg ml−1. Pigments also showed significant antimicrobial activity against Bacillus subtilis, Bacillus pumilus, Enterococcus faecalis, Bacillus cereus, Klebsiella pneumoniae, Alcaligenes faecalis, Pseudomonas geniculata, Enterococcus faecium, Aspergillus fumigatus, Aspergillus flavus, Fusarium solani, and Mucor spp., suggesting potential biomedical applications.

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Acknowledgements

Authors are thankful to Dr. J. Chris Blazier (Institute of Genome Sciences and Society, Texas A&M University) for editing the final manuscript.

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Authors and Affiliations

  1. Microbiology and Biotechnology Research Lab, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, 46000, Pakistan

    Anila Fariq & Azra Yasmin

  2. Department of Biotechnology, Fatima Jinnah Women University, Rawalpindi, 46000, Pakistan

    Anila Fariq & Azra Yasmin

  3. Department of Botany, University of Sargodha, Sargodha, Pakistan

    Muhammad Jamil

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  1. Anila Fariq
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  2. Azra Yasmin
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  3. Muhammad Jamil
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Correspondence to Azra Yasmin.

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Communicated by M. Moracci.

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Fariq, A., Yasmin, A. & Jamil, M. Production, characterization and antimicrobial activities of bio-pigments by Aquisalibacillus elongatus MB592, Salinicoccus sesuvii MB597, and Halomonas aquamarina MB598 isolated from Khewra Salt Range, Pakistan. Extremophiles 23, 435–449 (2019). https://doi.org/10.1007/s00792-019-01095-7

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  • DOI: https://doi.org/10.1007/s00792-019-01095-7

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