Improved production of bacteriorhodopsin from Halobacterium salinarum through direct amino acid supplement in the basal medium
Enhanced production and growth of Halobacterium salinarum are achieved by direct supplement of essential amino acids in the modified nutrient culture medium. As arginine (R) and aspartate (D) are the main amino acid sources for producing bacteriorhodopsin efficiently from Halobacterium salinarum, both individual and combined effects of these two amino acids (in different compositions) in the basal medium were studied. The BR production is enhanced by 83% on the eighth day (saturated) for all individual and combined amino acid supplements. Maximum production of 201 mg/l is observed for combined amino acid (R3D2)-supplemented culture which is 4.46-fold higher than the conventional culture growth from the basal medium. The obtained results suggest the efficient method to enhance BR production at low cost and thus, open up the possibility to utilize this potential biomolecule for various photonics applications which were earlier restricted due to the high cost of protein molecules.
KeywordsBacteriorhodopsin Amino acid Arginine aspartate Enhanced BR production
This work is the extension of a project funded by the Department of Science and Technology, Government of India under the DST-SERB Startup Grant Scheme (Grant no.SB/FTP/PS-038/2014). Mr. C. J. wishes to thank University Grants Commission, Government of India for providing UGC-SAP (RFMS) fellowship to carry out his research work (Grant. no. F.4-1/2006(BSR)/7-197/2007). Mehboobali Pannipara is thankful to Dean of Scientific Research, King Khalid University for financial support by grant number G. R. P-276-39.
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