Behavior of the extremophile green alga Coccomyxa melkonianii SCCA 048 in terms of lipids production and morphology at different pH values
- 102 Downloads
The extremophile green alga Coccomyxa melkonianii SCCA 048 was investigated to evaluate its ability to grow in culture media with different pH. Specifically, Coccomyxa melkonianii was sampled in the Rio Irvi river (Sardinia, Italy) which is severely polluted by heavy metals as a result of abandoned mining activities. In this study, the strain was cultivated in growth media where the pH was kept fixed at the values of 4.0, 6.8 and 8.0, respectively. During the investigation, a significant phenotypic plasticity of this strain was observed. The strain grew well in the pH range 4.0–8.0, while the optimal value for its growth was 6.8. Furthermore, maximum lipid contents of about 24 and 22 %wt were achieved at the end of cultivation when using pH 4.0 and 8.0, respectively. Finally, the analysis of fatty acid methyl esters (FAMEs) highlights the presence of suitable amounts of compounds which can be profitably exploited in the food, nutraceutical, and cosmetic industry. This aspect, coupled with the possibility of cultivating Coccomyxa melkonianii under extreme pH conditions in economic open ponds, makes this strain an interesting candidate for several biotechnological applications.
KeywordsCoccomyxa melkonianii SCCA 048 Extremophile algae pH response Fatty acids Morphology
The financial support of the COMISAR project (POR FESR 2014/2020 - Asse prioritario I “ricerca scientifica, sviluppo tecnologico e innovazione” Regione Autonoma della Sardegna, Italy) is gratefully acknowledged. One of us S.S. acknowledges the financial support obtained from the University of Cagliari during her Ph.D. program in Innovation Sciences and Technologies.
- Anitori RP (2012) Extremophiles: microbiology and biotechnology. Caister Academic Press, PooleGoogle Scholar
- Azov Y (1982) Effect of pH on inorganic carbon uptake in algal cultures inorganic carbon uptake in algal cultures. Appl Environ Microbiol 43:1300–1306Google Scholar
- Bischoff HW, Bold HC (1963) Phycological Studies IV. Some soil algae from enchanted rock and related algal species. University of Texas Publicatin No. 6318, Austin, pp 1–95Google Scholar
- Darienko T, Gustavs L, Eggert A et al. (2015) Evaluating the species boundaries of green microalgae (Coccomyxa, Trebouxiophyceae, Chlorophyta) using integrative taxonomy and DNA barcoding with further implications for the species identification in environmental samples. PLoS One 10:1–31CrossRefGoogle Scholar
- Iribar V (2004) Origin of neutral mine water in flooded underground mines: an appraisal using geochemical and hydrogeological methodologies. In: Proceedings, international mine water association symposium, Newcastle upon Tyne (University of Newcastle), pp 169–178Google Scholar
- Pasqualetti M, Tempesta S, Malavasi V et al (2015) Lutein production by Coccomyxa sp. SCCA048 isolated from a heavy metal-polluted river in Sardinia (Italy). J Environ Prot Ecol 16:1262–1272Google Scholar
- Scharer JM, Pettit CM, Kirkaldy JL, Bolduc L, Halbert BE, Chambers DB, Society for Mining, Metallurgy, and Exploration (2000) Leaching of metals from sulphide mine wastes at neutral pH. ICARD 2000 Proceedings from the fifth international conference on acid rock drainage, vol I. The Society, Littleton, Colo, pp 191–201Google Scholar