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Extremophiles

, Volume 22, Issue 6, pp 965–974 | Cite as

A metabolic and genomic assessment of sugar fermentation profiles of the thermophilic Thermotogales, Fervidobacterium pennivorans

  • Scott Wushke
  • Brian Fristensky
  • Xiang Li Zhang
  • Vic Spicer
  • Oleg V. Krokhin
  • David B. Levin
  • Matthew B. Stott
  • Richard Sparling
Original Paper

Abstract

A metabolic, genomic and proteomic assessment of Fervidobacterium pennivorans strains was undertaken to clarify the metabolic and genetic capabilities of this Thermotogales species. The type strain Ven5 originally isolated from a hot mud spa in Italy, and a newly isolated strain (DYC) from a hot spring at Ngatamariki, New Zealand, were compared for metabolic and genomic differences. The fermentation profiles of both strains on cellobiose generated similar major end products (acetate, alanine, glutamate, H2, and CO2). The vast majority of end products produced were redox neutral, and carbon balances were in the range of 95–115%. Each strain showed distinct fermentation profiles on sugar substrates. The genome of strain DYC was sequenced and shown to have high sequence similarity and synteny with F. pennivorans Ven5 genome, suggesting they are the same species. The unique genome regions in Ven5, corresponded to genes involved in the Entner–Doudoroff pathway confirming our observation of DYC’s inability to utilize gluconate. Genome analysis was able to elucidate pathways involved in production of the observed end-products with the exception of alanine and glutamate synthesis which were resolved with less clarity due to poor sequence identity and missing critical enzymes within the pathway, respectively.

Keywords

Thermophilic Fermentation Metabolism 

Notes

Acknowledgements

This research was supported by Genome Canada, through the Applied Genomics Research in Bioproducts or Crops (ABC) program for the grant titled, “Microbial Genomics for Biofuels and Co-Products from Biorefining Processes” and by the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant (RGPIN-06173-2014) held by Dr. Sparling. The authors wish to acknowledge and thank Ngāti Tahu-Ngāti Whaoa Runanga Trust for their enthusiasm for our research, and assistance in access and sampling of the Ngatamariki geothermal features; Ki ngā tāngata whenua, ngā mihi nui mō tō koutou manaaki i te kaupapa. Ngāti Tahu-Ngāti Whaoa are acknowledged as the having mana whenau (customary rights) over this strain and genome. Support for this work was provided by Geothermal Resources of New Zealand (GRN) Programme at GNS Science.

Supplementary material

792_2018_1053_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1110 kb)
792_2018_1053_MOESM2_ESM.pdf (315 kb)
Supplementary material 2 (PDF 315 kb)

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of ManitobaWinnipegCanada
  2. 2.Department Plant ScienceUniversity of ManitobaWinnipegCanada
  3. 3.Department Physics and AstronomyUniversity of ManitobaWinnipegCanada
  4. 4.Department of Internal Medicine and Manitoba Centre for Proteomics and Systems BiologyUniversity of ManitobaWinnipegCanada
  5. 5.Department of Biosystems EngineeringUniversity of ManitobaWinnipegCanada
  6. 6.Geomicrobiology Research GroupGNS ScienceWairakeiNew Zealand
  7. 7.School of Biological SciencesUniversity of CanterburyChristchurchNew Zealand

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