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Extremophiles

, Volume 22, Issue 6, pp 943–954 | Cite as

Characterization of protistan plankton diversity in ancient salt evaporation ponds located in a volcanic crater on the island Sal, Cape Verde

  • Feng Zhao
  • Sabine Filker
Original Paper

Abstract

Salinity is an important factor when exploring the limits known for life. Therefore, hypersaline systems have attracted much attention in recent years. In this study, we investigated the protistan diversity and community composition in two natural salt evaporation ponds (27–30% salinity) located in an ancient volcanic crater on the Cape Verde island Sal using high-throughput DNA sequencing. Our study revealed a broad range of protistan taxa and a high taxonomic diversity within the Ciliophora, Dinophyceae, and Chlorophyta. We detected a total of 23 Dinophyceae families, although Dinophyceae were generally considered to be only this diverse in aquatic environments of less than 10% salinity. Moreover, we uncovered a high degree of genetic novelty in this habitat. The mean similarity of all detected OTUs to previously described sequences was only 93.6%. These findings strongly dispute the traditional view that extreme hypersaline environments generally maintain low protistan diversity. A meta-analysis covering our and previously published data from other inland and coastal salt ponds clearly showed that our samples clustered according to salinity and not biogeography. This result further supports the claim that salinity is a major transition boundary for protistan communities, regardless of their biogeographic origin.

Keywords

Biogeography Diversity Halophiles High-throughput DNA sequencing Hypersaline environments Protists 

Notes

Acknowledgements

This research was funded by a grant from the TU Nachwuchsring to SF. Research of FZ was supported by the China Scholarship Council (CSC, No. 201604910395). Samples were collected during a research cruise with R/V Meteor (M118) funded by the DFG Senatskommission. We thank Thorsten Stoeck (University of Technology Kaiserslautern) for his help with sample collection. Data analysis was performed on the high-performance computing cluster “Elwetritsch” at the University of Kaiserslautern supported by an AHRP Grant to SF.

Supplementary material

792_2018_1050_MOESM1_ESM.xlsx (11 kb)
Supplementary material 1 (XLSX 11 kb)

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© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Marine Organism Taxonomy and Phylogeny, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Department of Molecular EcologyUniversity of Technology KaiserslauternKaiserslauternGermany

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