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Extremophiles

, Volume 22, Issue 6, pp 865–875 | Cite as

Potential of Lentibacillus sp. NS12IITR for production of lipids with enriched branched-chain fatty acids for improving biodiesel properties along with hydrocarbon co-production

  • Noopur Singh
  • Bijan Choudhury
Original Paper
  • 87 Downloads

Abstract

Hypersaline environment is inhabited by array of microbes which have the potential to produce industrially important products. This study explored biomass and lipid production potential of the halophilic bacterium, strain NS12IITR which was isolated from Sambhar Lake, Rajasthan. Sequencing and phylogenetic analysis revealed that the bacterium belonged to genus Lentibacillus. The salient feature of the isolate is its ability to accumulate total cellular lipid up to 18.9 ± 0.45% of dry cell weight. In addition, trans-esterification of extracted lipid yielded 77.6 ± 5.56% of total esters as methyl ester of branched-chain fatty acids (BCFAs). To assess the nature of extracted lipid, lipid sample was fractionated on the silicic acid column, which demonstrated that 49.03 ± 1.35% of the total lipids was neutral in nature. Trans-esterification of the neutral lipid fraction yielded 60.62 ± 4.88% of total esters as methyl ester of BCFAs. Methyl esters of BCFAs were present in trans-esterified products of neutral as well as polar lipid fractions. Furthermore, the isolate produced hydrocarbons both extracellularly (C10–C30) and intra-cellularly (C15–C28). The concentration of extracellular hydrocarbon (21.11 ± 0.78 mg/L) synthesized by strain NS12IITR is in close agreement with the yield reported from other hydrocarbon producing bacteria. This is hereby a first report on the co-production of lipids and hydrocarbon from a halophilic bacterium. The production of neutral lipid with high percentage of BCFAs and co-production of hydrocarbons makes the isolate NS12IITR a potential claimant for biofuel production.

Keywords

Lentibacillus Branched-chain fatty acids Hydrocarbon Anteiso-pentadecanoic acid Halophiles Biodiesel 

Abbreviations

BCFA

Branched-chain fatty acid

SCFA

Straight-chain fatty acid 

NaCl

Sodium chloride

KCl

Potassium chloride

MgSO4

Magnesium sulfate

KH2PO4

Potassium dihydrogen phosphate

FeSO4

Ferrous sulfate

FAME

Fatty acid methyl ester

HCl

Hydrochloric acid

GC–MS

Gas chromatography and mass spectrometry

μL

Micro liter

mL

Milliliter

M

Molar

BAME

Bacterial acid methyl ester

µg

Micro gram

g/L

Gram per liter

mg/L

Milligram per liter

SFA

Saturated fatty acid

NL

Neutral lipid

GL ± SL

Glycolipid and sphingolipid

PL

Phospholipid

TLC

Thin-layer chromatography

MnCl2

Manganese chloride

TAG

Triacylglyceride

DW

Distilled water

H2SO4

Sulphuric acid

MAGs

Monoacylglycerols

Chol

Cholesterol

1,2 DAGs

1,2 diacylglycerols

1,3 DAGs

1,3 diacylglycerols

FFAs

Free fatty acid

CE

Cholesterol esters

HAAL

High altitude Andean lake

TAE

Tris acetate EDTA

EDTA

Ethylene diamine tetraacetic acid

pH

Potential of hydrogen

HC

Hydrocarbon

FAAE

Fatty acid acyl ester

PCR

Polymerase chain reaction

Notes

Acknowledgements

The authors gratefully acknowledge the financial support IIT Roorkee and Govt. of India for providing research fellowship.

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiotechnologyIIT RoorkeeRoorkeeIndia

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