Heavy metal tolerance in marine strains of Yarrowia lipolytica
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Heavy metal tolerance of two marine strains of Yarrowia lipolytica was tested on solid yeast extract peptone dextrose agar plates. Based on minimum inhibitory concentration esteems, it is inferred that the two strains of Y. lipolytica were tolerant to heavy metals such as Pb(II), Cr(III), Zn(II), Cu(II), As(V), and Ni(II) ions. The impact of various heavy metal concentrations on the growth kinetics of Y. lipolytica was likewise assessed. With increased heavy metal concentration, the specific growth rate was reduced with delayed doubling time. Furthermore, biofilm development of both yeasts on the glass surfaces and in microtitre plates was assessed in presence of different heavy metals. In microtitre plates, a short lag phase of biofilm formation was noticed without the addition of heavy metals in yeast nitrogen base liquid media. A lag phase was extended over increasing metal concentrations of media. Heavy metals like Cr(VI), Cd(II), and As(V) are contrastingly influenced on biofilms’ formation of microtitre plates. Other heavy metals did not much influence on biofilms development. Thus, biofilm formation is a strategy of Y. lipolytica under stress of heavy metals has significance in bioremediation process for recovery of heavy metals from contaminated environment.
KeywordsYarrowia lipolytica Metal tolerance MIC Lag phase Specific growth rates
All authors are thankful to CSIR, UGC, New Delhi, India and BCUD, Savitribai Phule Pune University, Pune, India for financial support.
Compliance with ethical standards
Conflict of interest
All authors declare that there is no conflict of interest.
- Das N, Lakshmi V, Basak G, Salam JA, Abigail MEA (2012) Application of biofilms on remediation of pollutants—an overview. J Microbiol Biotechnol Res 2:783–790Google Scholar
- Ezzouhri L, Castro E, Moya M, Espinola F, Lairini K (2009) Heavy metal tolerance of filamentous fungi isolated from polluted sites in Tangier, Morocco. Afr J Microbiol Res 3:35–48Google Scholar
- Ford TE (2000) Response of marine microbial communities to anthropogenic stress. Stress in marine communities. J Aquat Ecosyst Stress Recovery 7:75–89 (Attrill MJ (ed)) Google Scholar
- Gonye ER, Jones GE (1973) An ecological survey of open ocean and estuarine microbial populations. II. The oligodynamic effect of nickel on marine bacteria. In: Stevenson HL (ed) Marine microbial ecology. University of South Carolina Press, Columbia, pp 243–257Google Scholar
- Hagler AN, Mendocs-Hageler LC (1981) Yeasts from marine and estuarine waters with different levels of pollution in the State of Rio de Janeiro, Brazil. Appl Environ Microbiol 416:173–178Google Scholar
- Hosam EAF, Hamuda B, Tóth N (2012) Functioning of divalent alkaline metal on yeast multiplication in heavy metal contaminated soil. Tájökológiai Lapok 10:195–208Google Scholar
- Ilyas S, Rehman A, Ilyas Q (2017) Heavy metals induced oxidative stress in multi-metal tolerant yeast, Candida sp. PS33 and its capability to uptake heavy metals from wastewater. Pakistan. J Zool 49(3):769–775Google Scholar
- Islam MS, Saha AK, Mosaddeque HQM, Amin MR, Islam MM (2008) In vitro studies on the reaction of fungi Trichoderma to different herbicides used in tea plantation. Int J Sustain Crop Prod 3(5):27–30Google Scholar
- Jones GE (1970) Metal organic complexes formed by marine bacteria. In: Hood DW (ed) Organic matter in natural waters. Inst. Mar. Sci. Occas. Publ. no. 1. University of Alaska Press, College, Alaska, pp 301–320Google Scholar
- Ksheminska H, Jaglarz A, Fedorovych D, Babyak L, Yanovych D, Kaszycki P, Koloczek H (2003) Bioremediation of chromium by the yeast Pichia guilliermondii: toxicity and accumulation of Cr(III) and Cr(VI) and the influence of riboflavin on Cr tolerance. Microbiol Res 158:59–67CrossRefPubMedGoogle Scholar
- Pasternakiewicz A (2006) The growth of Saccharomyces cerevisiae yeast in cadminum enriched medium. Acta Sci Pol Technol Aliment 5:39–46Google Scholar
- Strouhal M, Kizek R, Vacek J, Trnkova L, Nemec M (2003) Electrochemical study of heavy metals and metallothionein in yeast Yarrowia lipolytica. Bioelectronics 60:29–36Google Scholar
- Urnau K, Orlowska E, Ryszka P, Zubek S, Anielska T, Gawronski S, Jurkiewicz A (2006) Role of mycorrhizal fungi in phytoremediation and toxicity monitoring of heavy metal rich industrial wastes in southern Poland. Soil Water Pollut Monit Prot Remediat 3:533Google Scholar