Yeasts from sub-Antarctic region: biodiversity, enzymatic activities and their potential as oleaginous microorganisms
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Various microbial groups are well known to produce a range of extracellular enzymes and other secondary metabolites. However, the occurrence and importance of investment in such activities have received relatively limited attention in studies of Antarctic soil microbiota. Sixty-one yeasts strains were isolated from King George Island, Antarctica which were characterized physiologically and identified at the molecular level using the D1/D2 region of rDNA. Fifty-eight yeasts (belonging to the genera Cryptococcus, Leucosporidiella, Rhodotorula, Guehomyces, Candida, Metschnikowia and Debaryomyces) were screened for extracellular amylolytic, proteolytic, esterasic, pectinolytic, inulolytic xylanolytic and cellulolytic activities at low and moderate temperatures. Esterase activity was the most common enzymatic activity expressed by the yeast isolates regardless the assay temperature and inulinase was the second most common enzymatic activity. No cellulolytic activity was detected. One yeast identified as Guehomyces pullulans (8E) showed significant activity across six of seven enzymes types tested. Twenty-eight yeast isolates were classified as oleaginous, being the isolate 8E the strain that accumulated the highest levels of saponifiable lipids (42 %).
KeywordsBioenergetics Enzymes Psychrophiles
This work was supported by grants from Agencia Nacional de Investigación e Innovación (ANII, FSE 102780), Instituto Antártico Uruguayo (IAU), Pedeciba, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP 112-201101-00662) and Agencia Nacional de PromociónCientífica y Tecnológica (PICT 2014-1655).
- Connell LB, Redman R, Rodriguez R, Barrett A, Iszard M, Fonseca Á (2010) Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica. Int J Syst Evol Microbiol 60:1466–1472. doi: 10.1099/ijs.0.015412-0 CrossRefPubMedGoogle Scholar
- Di Rienzo JACF, Balzarini MG, Gonzalez L, Tablada M, Robledo CW (2009) InfoStat versión 2009. Universidad Nacional de Córdoba, ArgentinaGoogle Scholar
- Frisvad JC (2008) Fungi in cold ecosystems. In: Psychrophiles: from biodiversity to biotechnology, pp 137–156. doi: 10.1007/978-3-540-74335-4_9
- Garay LA et al (2016) Eighteen new oleaginous yeast species. J Ind Microbiol Biotechnol: 1–14Google Scholar
- Hammer Ø, Harper DAT, Ryan PD (2001) Past: paleontological statistics software package for education and data analysis Palaeontol Electron 4:XIX–XXGoogle Scholar
- Margesin R, Fonteyne PA, Schinner F, Sampaio JP (2007) Rhodotorula psychrophila sp. nov., Rhodotorula psychrophenolica sp. nov. and Rhodotorula glacialis sp. nov., novel psychrophilic basidiomycetous yeast species isolated from alpine environments. Int J Syst Evol Microbiol 57:2179–2184. doi: 10.1099/ijs.0.65111-0 CrossRefPubMedGoogle Scholar
- Mohamed Hatha AA, Rahiman Mujeeb RK, Krishnan KP, Saramma AV, Saritha G, Lal D (2013) Characterisation and bioprospecting of cold adapted yeast from water samples of Kongsfjord. Nor Arct Indian J Mar Sci 42:458–465Google Scholar
- Pulicherla K, Ghosh M, Kumar PS, Rao KS (2012) Psychrozymes—the next generation industrial enzymes. J Mar Sci Res Dev 2011Google Scholar
- Shivaji S, Prasad G (2009) Antarctic yeasts: biodiversity and potential applications. In: Yeast biotechnology: diversity and applications. Springer, New York, pp 3–18Google Scholar
- Vishniac HS (2006) Yeast biodiversity in the Antarctic. In: Biodiversity and ecophysiology of yeasts. Springer, New York, pp 419–440Google Scholar
- Zhang S, Skerker JM, Rutter CD, Maurer MJ, Arkin AP, Rao CV (2015) Engineering Rhodosporidium toruloides for increased lipid production. Biotechnol BioengGoogle Scholar