Extremophiles

, Volume 20, Issue 1, pp 27–36 | Cite as

Transcriptional regulation of dimethyl sulfoxide respiration in a haloarchaeon, Haloferax volcanii

  • Qiuzi Qi
  • Yoshiyasu Ito
  • Katsuhiko Yoshimatsu
  • Taketomo Fujiwara
Original Paper
  • 183 Downloads

Abstract

The halophilic euryarchaeon Haloferax volcanii can grow anaerobically by DMSO respiration. DMSO reductase was induced by DMSO respiration not only under anaerobic growth conditions but also in denitrifying cells of H. volcanii. Deletion of the dmsR gene, encoding a putative regulator for the DMSO reductase, resulted in the loss of anaerobic growth by DMSO respiration. Reporter experiments revealed that only the anaerobic condition was essential for transcription of the dmsEABCD genes encoding DMSO reductase and that transcription was enhanced threefold by supplementation of DMSO. In the ∆dmsR mutant, transcription of the dmsEABCD genes induced by the anaerobic condition was not enhanced by DMSO, suggesting that DmsR is a DMSO-responsive regulator. Transcriptions of the dmsR and mgd genes for Mo-bisMGD biosynthesis were regulated in the same manner as the dmsEABCD genes. These results suggest that the genetic regulation of DMSO respiration in H. volcanii is controlled by at least two systems: one is the DMSO-responsive DmsR, and the other is an unknown anaerobic regulator.

Keywords

Haloferax volcanii DMSO reductase Anaerobic respiration DmsR Transcription regulator 

Abbreviations

ANOVA

Analysis of variance

BgaH

Halophilic β-galactosidase

DMSO

Dimethyl sulfoxide

DMS

Dimethyl sulfide

Mo-bisMGD

Bis-molybdopterin guanine dinucleotide

MPT

Molybdopterin

MV

Methyl viologen

ORF

Open reading frame

PyrE2

Orotate:phosphoribosyl transferase

Supplementary material

792_2015_794_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1126 kb)

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Copyright information

© Springer Japan 2015

Authors and Affiliations

  • Qiuzi Qi
    • 1
  • Yoshiyasu Ito
    • 2
  • Katsuhiko Yoshimatsu
    • 3
  • Taketomo Fujiwara
    • 1
    • 2
  1. 1.Department of Environment and Energy Systems, Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan
  2. 2.Department of Biological Science, Faculty of ScienceShizuoka UniversityShizuokaJapan
  3. 3.Research Institute of Green Science and TechnologyShizuoka UniversityShizuokaJapan

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