, Volume 19, Issue 2, pp 373–382 | Cite as

A unique glyceryl diglycoside identified in the thermophilic, radiation-resistant bacterium Rubrobacter xylanophilus

  • Pedro Lamosa
  • Eva C. Lourenço
  • Filipa d’Avó
  • Ana Nobre
  • Tiago M. Bandeiras
  • Milton S. da Costa
  • M. Rita Ventura
  • Helena Santos
Original Paper


The solute pool of the actinobacterium Rubrobacter xylanophilus has been investigated as a function of the growth temperature and concentration of NaCl in the medium (Empadinhas et al. Extremophiles 11: 667–673, 2007). Changing the carbon source from glucose to maltose in a minimal growth medium led to the accumulation of an unknown organic compound whose structure was investigated by NMR and confirmed by chemical synthesis in the present study as: (2R)-2-(1-O-α-d-mannopyranosyl)-3-(1-O-α-d-glucopyranosyl)-d-glycerate (MGlyG). In addition to this newly identified diglycoside, the solute pool of R. xylanophilus included trehalose, mannosylglycerate, di-myo-inositol phosphate and di-N-acetyl-glucosamine phosphate. The structure of MGlyG was established by NMR and confirmed by chemical synthesis. The availability of g-amounts of the synthetic material allowed us to perform stabilization tests on three model enzymes (malate dehydrogenase, staphylococcal nuclease, and lysozyme), and compare the efficacy of MGlyG with other natural glyceryl glycosides, such as α-d-mannosyl-d-glycerate, α-d-glucosyl-d-glycerate and α-d-glucosyl-(1 → 6)-α-d-glucosyl-(1 → 2)-d-glycerate.


Rubrobacter xylanophilus Compatible solute Protein stabilization Glyceryl glycosides 



The NMR spectrometers are part of The National NMR Facility (RECI/BBB-BQB/0230/2012), supported by Fundação para a Ciência e a Tecnologia. E.L. acknowledges Fundação para a Ciência e a Tecnologia for a PhD grant SFRH/47702/2008. We thank Ana Isabel Mingote for her assistance in solute purification and Tm determination.

Supplementary material

792_2014_723_MOESM1_ESM.doc (354 kb)
Supplementary material 1 (DOC 353 kb)


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Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Pedro Lamosa
    • 1
  • Eva C. Lourenço
    • 1
  • Filipa d’Avó
    • 2
  • Ana Nobre
    • 2
  • Tiago M. Bandeiras
    • 1
    • 3
  • Milton S. da Costa
    • 4
  • M. Rita Ventura
    • 1
  • Helena Santos
    • 1
  1. 1.Instituto de Tecnologia Química e BiológicaUniversidade Nova de LisboaOeirasPortugal
  2. 2.CNC-Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbraPortugal
  3. 3.IBET-Instituto de Biologia Experimental e TecnológicaOeirasPortugal
  4. 4.Department of Life SciencesUniversity of CoimbraCoimbraPortugal

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