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Extremophiles

, Volume 19, Issue 1, pp 1–15 | Cite as

Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 °C and hypersaline sediments of a high Arctic permafrost spring

  • Guillaume Lamarche-Gagnon
  • Raven Comery
  • Charles W. Greer
  • Lyle G. WhyteEmail author
SPECIAL ISSUE: ORIGINAL PAPER 10th International Congress on Extremophiles
Part of the following topical collections:
  1. 10th International Congress on Extremophiles

Abstract

The lost hammer (LH) spring perennially discharges subzero hypersaline reducing brines through thick layers of permafrost and is the only known terrestrial methane seep in frozen settings on Earth. The present study aimed to identify active microbial communities that populate the sediments of the spring outlet, and verify whether such communities vary seasonally and spatially. Microcosm experiments revealed that the biological reduction of sulfur compounds (SR) with hydrogen (e.g., sulfate reduction) was potentially carried out under combined hypersaline and subzero conditions, down to −20 °C, the coldest temperature ever recorded for SR. Pyrosequencing analyses of both 16S rRNA (i.e., cDNA) and 16S rRNA genes (i.e., DNA) of sediments retrieved in late winter and summer indicated fairly stable bacterial and archaeal communities at the phylum level. Potentially active bacterial and archaeal communities were dominated by clades related to the T78 Chloroflexi group and Halobacteria species, respectively. The present study indicated that SR, hydrogenotrophy (possibly coupled to autotrophy), and short-chain alkane degradation (other than methane), most likely represent important, previously unaccounted for, metabolic processes carried out by LH microbial communities. Overall, the obtained findings provided additional evidence that the LH system hosts active communities of anaerobic, halophilic, and cryophilic microorganisms despite the extreme conditions in situ.

Keywords

Cryophile Halophile Sulfur/sulfate reduction Microbial ecology Anaerobic activity 16S rRNA 

Abbreviations

cmbs

cm below the sediment surface

SR

Sulfur reduction, (bio)chemical reduction of a sulfur compound (e.g., sulfate reduction)

SRR

Sulfide release rates; the rates of sulfide (H2S) production resulting from SR

Notes

Acknowledgments

We acknowledge the following funding organizations for financial support: the Fond québécois de recherche nature et technologies (FQRNT), the Canadian Astrobiology Training Program (NSERC CREATE CATP), the Northern Science Training Program (NSTP), as well as the Polar and Continental Shelf Project (PCSP) for logistical support in the field.

Supplementary material

792_2014_703_MOESM1_ESM.pdf (820 kb)
Supplementary material 1 (PDF 820 kb)

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Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Guillaume Lamarche-Gagnon
    • 1
  • Raven Comery
    • 1
  • Charles W. Greer
    • 2
  • Lyle G. Whyte
    • 1
    Email author
  1. 1.Department of Natural Resource Sciences (NRS)McGill UniversityMontrealCanada
  2. 2.National Research Council CanadaMontrealCanada

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