Extremophiles

, Volume 19, Issue 1, pp 183–188 | Cite as

Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia

  • Fatma Ben Aissa
  • Anne Postec
  • Gaël Erauso
  • Claude Payri
  • Bernard Pelletier
  • Moktar Hamdi
  • Marie-Laure Fardeau
  • Bernard Ollivier
Original Paper

Abstract

A novel anaerobic, alkaliphilic, Gram-positive staining bacterium was isolated from a hydrothermal chimney in the Prony Bay, New Caledonia. This strain designated FatMR1T grew at temperatures from 20 to 55 °C (optimum 37 °C) and at pH between 7.5 and 10.5 (optimum 8.8–9). NaCl is not required for growth (optimum 0.2–0.5 %), but is tolerated up to 3 %. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite are not used as terminal electron acceptors. Strain FatMR1T fermented pyruvate, yeast extract, peptone and biotrypcase and used fructose as the only sugar. The main fermentation products from fructose and proteinaceous compounds (e.g. peptone and biotrypcase) were acetate, H2 and CO2. Crotonate was disproportionated to acetate and butyrate. The predominant cellular fatty acids were C14:0 and C16:0. The G + C content of the genomic DNA was 37.1 mol %. On the basis of phylogenetic, genetic, and physiological properties, strain FatMR1T (=DSM 25890T, =JCM 18390T) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as a novel species of the genus Alkaliphilus, A. hydrothermalis sp. nov.

Keywords

Firmicutes Alkaliphilus hydrothermalis Alkaliphilic Anaerobic Alkaline hydrothermal vents Serpentinization New Caledonia 

References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410PubMedCrossRefGoogle Scholar
  2. Balch WE, Fox GE, Magrum RJ, Wolfe RS (1979) Methanogens: re-evaluation of a unique biological group. Microbiol Rev 43:260–296PubMedCentralPubMedGoogle Scholar
  3. Ben Aissa F, Postec A, Erauso G, Payri C, Pelletier B, Hamdi M, Ollivier B, Fardeau ML (2014) Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney. Int J Syst Evol Microbiol 64:1160–1165PubMedCrossRefGoogle Scholar
  4. Brazelton WJ, Schrenk MO, Kelley DS, Baross JA (2006) Methane-and sulfur-metabolizing microbial communities dominate the Lost City hydrothermal field ecosystem. Appl Environ Microbiol 72:6257–6270PubMedCentralPubMedCrossRefGoogle Scholar
  5. Brazelton WJ, Ludwig KA, Sogin ML, Andreishcheva EN, Kelley DS, Shen CC, Edwards RL, Baross JA (2010) Archaea and bacteria with surprising microdiversity show shifts in dominance over 1,000-year time scales in hydrothermal chimneys. Proc Natl Acad Sci USA 107:1612–1617PubMedCentralPubMedCrossRefGoogle Scholar
  6. Cao X, Liu X, Dong X (2003) Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. Int J Syst Evol Microbiol 53:971–975PubMedCrossRefGoogle Scholar
  7. Cashion P, Holder-Franklin MA, McCully J, Franklin M (1977) A rapid method for the base ratio determination of bacterial DNA. Annal Biochem 81:461–466CrossRefGoogle Scholar
  8. Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J, Garcia P, Cai J, Hippe H, Farrow JAE (1994) The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826PubMedCrossRefGoogle Scholar
  9. Cord-Ruwisch R (1985) A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36CrossRefGoogle Scholar
  10. De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142PubMedCrossRefGoogle Scholar
  11. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucl Acids Res 32:1792–1797PubMedCentralPubMedCrossRefGoogle Scholar
  12. Fardeau ML, Ollivier B, Patel BK, Magot M, Thomas P, Rimbault A, Rocchiccioli F, Garcia JL (1997) Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019PubMedCrossRefGoogle Scholar
  13. Fardeau ML, Magot M, Patel BK, Thomas P, Garcia JL, Ollivier B (2000) Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oil field water. Int J Syst Evol Microbiol 6:2141–2149CrossRefGoogle Scholar
  14. Hungate RE (1969) A roll-tube method for the cultivation of strict anaerobes. Methods Microbiol 3B:117–132CrossRefGoogle Scholar
  15. Huß VA, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192PubMedCrossRefGoogle Scholar
  16. Kelley DS, Karson JA, Blackman DK, Früh-Green GL, Butterfield DA, Lilley MD, Olson EJ, Schrenk MO, Roe KK, Lebon GT, Rivizzigno P, AT3-60 Shipboard Party (2001) An off-axis hydrothermal vent field near the Mid-Atlantic Ridge at 30 degrees N. Nature 412:145–149PubMedCrossRefGoogle Scholar
  17. Khelifi N, Ben Romdhane E, Hedi A, Postec A, Fardeau M-L, Hamdi M, Tholozan J-L, Ollivier B, Hirschler-Réa A (2010) Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. Extremophiles 14:297–304PubMedCrossRefGoogle Scholar
  18. Kuykendall LD, Roy MA, O´Neil JJ, Devine TE (1988) Fatty acids, antibiotic resistance, and desoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38:358–361CrossRefGoogle Scholar
  19. Launay J, Fontes JC (1985) Les sources thermales de Prony (Nouvelle-Calédonie) et leurs précipités chimiques: exemple de formation de brucite primaire. Géologie de la France 83–100Google Scholar
  20. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  21. Miller LT (1982) Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586PubMedCentralPubMedGoogle Scholar
  22. Monnin C, Chavagnac V, Boulart C, Ménez B et al (2014) The low temperature hyperalkaline hydrothermal system of the Prony bay (New Caledonia). Biogeosciences Discuss 11(4):6221–6267CrossRefGoogle Scholar
  23. Pelletier B, Chevillon C, Menou J, Butscher J, Folcher E et al. (2006) Plongées, forage et cartographie Baie du Prony et Banc Gail, lagon Sud de Nouvelle-Calédonie, campagne 2005-NC-PL du N.O. ALIS 13–17 Juin 2005 et cartographie baie du Prony et canal Woodin N.O. ALIS 25–26 September 2005: Nouméa IRD Sept. 2006. Missions Sci Terre, Géol-Géophys 70:44Google Scholar
  24. Quéméneur M, Bes M, Postec A, Mei N et al. (2014) Spatial distribution of microbial communities in the shallow submarine alkaline hydrothermal field of the Prony Bay, New Caledonia. Environ Microbiol Rep (in press)Google Scholar
  25. Russell MJ, Hall AJ, Martin W (2010) Serpentinization and its contribution to the energy for the emergence of life. Geobiol 8:355–371CrossRefGoogle Scholar
  26. Schrenk MO, Kelley DS, Bolton SA, Baross JA (2004) Low archaeal diversity linked to subseafloor geochemical processes at the Lost City hydrothermal field, Mid-Atlantic Ridge. Environ Microbiol 6:1086–1095PubMedCrossRefGoogle Scholar
  27. Schrenk MO, Brazelton WJ, Lang SQ (2013) Serpentinization, carbon, and deep life. Rev Mineral Geochem 75:575–606CrossRefGoogle Scholar
  28. Takai K, Moser DP, Onstott TC, Spoelstra N, Pfiffner SM, Dohnalkova A, Fredrickson JK (2001) Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 51:1245–1256PubMedGoogle Scholar
  29. Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci (USA) 101:11030–11035CrossRefGoogle Scholar
  30. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRefGoogle Scholar
  31. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler P, Krichevsky MI, Moore LH, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  32. Wu XY, Shi KL, Xu XW, Wu M, Oren A, Zhu XF (2010) Alkaliphilus halophilus sp. nov., a strictly anaerobic and halophilic bacterium isolated from a saline lake, and emended description of the genus Alkaliphilus. Int J Syst Evol Microbiol 60:2898–2902PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Fatma Ben Aissa
    • 1
    • 2
  • Anne Postec
    • 1
  • Gaël Erauso
    • 1
  • Claude Payri
    • 3
  • Bernard Pelletier
    • 3
  • Moktar Hamdi
    • 2
  • Marie-Laure Fardeau
    • 1
  • Bernard Ollivier
    • 1
  1. 1.IRD, Mediterranean Institute of Oceanography (MIO)CNRS/INSU, Aix-Marseille Université, Université du Sud Toulon-VarMarseille Cedex 09France
  2. 2.Laboratoire d’Ecologie et de Technologie MicrobienneInstitut National des Sciences Appliquées et de Technologie, Centre Urbain NordTunis CedexTunisia
  3. 3.Institut pour la Recherche et le Développement (IRD) Centre de NouméaNouméaNouvelle Calédonie

Personalised recommendations