, Volume 18, Issue 3, pp 617–627 | Cite as

Correlation between the optimal growth pressures of four Shewanella species and the stabilities of their cytochromes c 5

  • Misa Masanari
  • Satoshi Wakai
  • Manabu Ishida
  • Chiaki Kato
  • Yoshihiro SambongiEmail author
Original Paper


Shewanella species live widely in deep-sea and shallow-water areas, and thus grow piezophilically and piezosensitively. Piezophilic and psychrophilic Shewanella benthica cytochrome c 5 (SB cytc 5) was the most stable against guanidine hydrochloride (GdnHCl) and thermal denaturation, followed by less piezophilic but still psychrophilic Shewanella violacea cytochrome c 5 (SV cytc 5). These two were followed, as to stability level, by piezosensitive and mesophilic Shewanella amazonensis cytochrome c 5 (SA cytc 5), and piezosensitive and psychrophilic Shewanella livingstonensis cytochrome c 5 (SL cytc 5). The midpoint GdnHCl concentrations of SB cytc 5, SV cytc 5, SL cytc 5, and SA cytc 5 correlated with the optimal growth pressures of the species, the correlation coefficient value being 0.93. A similar trend was observed for thermal denaturation. Therefore, the stability of each cytochrome c 5 is related directly to its host’s optimal growth pressure. Phylogenetic analysis indicated that Lys-37, Ala-41, and Leu-50 conserved in piezosensitive SL cytc 5 and SA cytc 5 are ancestors of the corresponding residues in piezophilic SB cytc 5 and SV cytc 5, Gln, Thr, and Lys, respectively, which might have been introduced during evolution on adaption to environmental pressure. The monomeric Shewanella cytochromes c 5 are suitable tools for examining protein stability with regard to the optimal growth pressures of the source species.


Correlation Cytochrome c5 Growth pressure Protein stability Shewanella 


SA cytc5

Shewanella amazonensis cytochrome c 5

SB cytc5

Shewanella benthica cytochrome c 5

SL cytc5

Shewanella livingstonensis cytochrome c 5

SV cytc5

Shewanella violacea cytochrome c 5



This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (No. 20118005) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by the Cooperative Research Program of the “Network of Joint Research Center for Materials and Devices”.


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Copyright information

© Springer Japan 2014

Authors and Affiliations

  • Misa Masanari
    • 1
  • Satoshi Wakai
    • 2
  • Manabu Ishida
    • 3
  • Chiaki Kato
    • 4
  • Yoshihiro Sambongi
    • 1
    Email author
  1. 1.Graduate School of Biosphere ScienceHiroshima UniversityHigashi-HiroshimaJapan
  2. 2.Organization of Advanced Science and TechnologyKobe UniversityKobeJapan
  3. 3.Top Runner Incubation Center for Academia-Industry Fusion, Department of BioengineeringNagaoka University of TechnologyNagaokaJapan
  4. 4.Institute of BiogeosciencesJapan Agency for Marine-Earth Science and TechnologyYokosukaJapan

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